Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Cytotoxicity of portoamides in human cancer cells and analysis of the molecular mechanisms of action
CIIMAR, Portugal.
CIIMAR, Portugal.
CIIMAR, Portugal.
CIIMAR, Portugal.
Show others and affiliations
2017 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 12, no 12, article id e0188817Article in journal (Refereed) Published
Abstract [en]

Portoamides are cyclic peptides produced and released by the cyanobacterial strain Phormidium sp. presumably to interfere with other organisms in their ecosystems (" allelopathy"). Portoamides were previously demonstrated to have an antiproliferative effect on human lung carcinoma cells, but the underlying mechanism of this activity has not been described. In the present work, the effects of portoamides on proliferation were examined in eight human cancer cell lines and two non-carcinogenic cell lines, and major differences in sensitivities were observed. To generate hypotheses with regard to molecular mechanisms of action, quantitative proteomics using 2D gel electrophoresis and MALDI-TOF/ TOF were performed on the colon carcinoma cell line HT-29. The expression of proteins involved in energy metabolism (mitochondrial respiratory chain and pentose phosphate pathway) was found to be affected. The hypothesis of altered energy metabolism was tested in further experiments. Exposure to portoamides resulted in reduced cellular ATP content, likely due to decreased mitochondrial energy production. Mitochondrial hyperpolarization and reduced mitochondrial reductive capacity was observed in treated cells. Furthermore, alterations in the expression of peroxiredoxins (PRDX4, PRDX6) and components of proteasome subunits (PSB4, PSA6) were observed in portoamide-treated cells, but these alterations were not associated with detectable increases in oxidative stress. We conclude that the cytotoxic activity of portoamides is associated with disturbance of energy metabolism, and alterations in mitochondrial structure and function.

Place, publisher, year, edition, pages
PUBLIC LIBRARY SCIENCE , 2017. Vol. 12, no 12, article id e0188817
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:liu:diva-143908DOI: 10.1371/journal.pone.0188817ISI: 000417337800041PubMedID: 29216224OAI: oai:DiVA.org:liu-143908DiVA, id: diva2:1170039
Note

Funding Agencies|Structured Program of R&D&I INNOVMAR - Innovation and Sustainability in the Management and Exploitation of Marine Resources - Northern Regional Operational Program (NORTE) through the European Regional Development Fund (ERDF) [NORTE-01-0145-FEDER-000035]; national funds (FCT, Foundation for Science and Technology) [UID/Multi/04423/2013, UID/BIM/04501/2013, UID/IC/00051/2013]; RNEM (National Mass Spectrometry Network); FCT [IF/01358/2014, SFRH/BPD/112287/2015]

Available from: 2018-01-02 Created: 2018-01-02 Last updated: 2018-01-26

Open Access in DiVA

fulltext(9218 kB)6 downloads
File information
File name FULLTEXT01.pdfFile size 9218 kBChecksum SHA-512
062335eb1971ff2b84a05d53b822da96243138ac4cae807d11870146c588bb9eba3a36f7732982987af6459d799870bd8d6a52a8f79fe6eed933d857b2eb5c3c
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Linder, Stig
By organisation
Division of Drug ResearchFaculty of Medicine and Health Sciences
In the same journal
PLoS ONE
Cell Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 6 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 5 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf