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Nascent RNA sequencing of unperturbed newly divided cells
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre. University of Michigan, Department of Radiation Oncology. (Ljungman Lab)
2017 (English)Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
Abstract [en]

Establishing a definitive cell cycle progression has been one of the fundamental aims of cellular biology. Its importance lies in gaining insight into the basic processes of life as well as the functions of mutant cell cycle pathways in promoting cancer by replication deficiencies and loss of checkpoint control. Currently used methods to control cell cycle and synchronize cells, function by halting cell cycle progression. Such harsh methods are detrimental to the cell and insufficient to provide an accurate reflection of the cell cycle. This study focused on replicating and confirming the efficiency of a technique developed by Helmstetter, called the “Baby Machine,” that can produce new born cells with little to no perturbations. Using this in conjunction with a short pulse RNA labelling technique, called Bru-seq, allowed the capture and RNA sequencing of synchronized cells and its nascent RNA. Here we show the first glimpse into the transcriptional profile of newly divided cells as well as novel rapid exon splicing and transcription read-through processes.

Place, publisher, year, edition, pages
2017. , p. 36
Keyword [en]
cell cycle, cell synchronization, nascent RNA sequencing
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:uu:diva-335466OAI: oai:DiVA.org:uu-335466DiVA, id: diva2:1163025
External cooperation
University of Michigan, Department of Radiation Oncology
Educational program
Master Programme in Molecular Biotechnology
Presentation
2017-10-25, 16:00 (English)
Supervisors
Examiners
Available from: 2017-12-06 Created: 2017-12-05 Last updated: 2017-12-06Bibliographically approved

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Parks, Thesis(2246 kB)24 downloads
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Cell Biology

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CiteExportLink to record
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Citation style
  • apa
  • ieee
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  • en-US
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Output format
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