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Profiling of Saccharomyces cerevisiae transcription factors for engineering the resistance of yeast to lignocellulose-derived inhibitors in biomass conversion
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
2017 (English)In: Microbial Cell Factories, ISSN 1475-2859, E-ISSN 1475-2859, Vol. 16, article id 199Article in journal (Refereed) Published
Abstract [en]

Background: Yeast transcription factors (TFs) involved in the regulation of multidrug resistance (MDR) were investigated in experiments with deletion mutants, transformants overexpressing synthetic genes encoding TFs, and toxic concentrations of lignocellulose-derived substances added to cultures as complex mixtures or as specific compounds, viz. coniferyl aldehyde, 5-hydroxymethylfurfural, and furfural. Results: In the presence of complex mixtures of toxic substances from spruce wood, transformants overexpressing YAP1 and STB5, TFs involved in oxidative stress response, exhibited enhanced relative growth rates amounting to 4.589 +/- 0.261 and 1.455 +/- 0.185, respectively. Other TFs identified as important for resistance included DAL81, GZF3, LEU3, PUT3, and WAR1. Potential overlapping functions of YAP1 and STB5 were investigated in experiments with permutations of deletions and overexpression of the two genes. YAP1 complemented STB5 with respect to resistance to 5-hydroxymethylfurfural, but had a distinct role with regard to resistance to coniferyl aldehyde as deletion of YAP1 rendered the cell incapable of resisting coniferyl aldehyde even if STB5 was overexpressed. Conclusions: We have investigated 30 deletion mutants and eight transformants overexpressing MDR transcription factors with regard to the roles the transcription factors play in the resistance to toxic concentrations of lignocel-lulose-derived substances. This work provides an overview of the involvement of thirty transcription factors in the resistance to lignocellulose-derived substances, shows distinct and complementary roles played by YAP1 and STB5, and offers directions for the engineering of robust yeast strains for fermentation processes based on lignocellulosic feedstocks.

Place, publisher, year, edition, pages
BioMed Central, 2017. Vol. 16, article id 199
Keyword [en]
Lignocellulosic biomass conversion, Saccharomyces cerevisiae, Multidrug resistance, Transcription ctors, STB5, YAP1, LAVEAU T, 1994, MOLECULAR & GENERAL GENETICS, V244, P501 cau-Danila A, 2003, JOURNAL OF BIOLOGICAL CHEMISTRY, V278, P52641 i Z, 1998, MOLECULAR MICROBIOLOGY, V29, P1307 reira Clara, 2007, MOLECULAR MICROBIOLOGY, V66, P571 innen Steve, 2017, MICROBIAL CELL FACTORIES, V16, mougrand N, 2004, FEMS YEAST RESEARCH2nd International Meeting on Yeast Apoptosis, SEP, 2003, olenice, SLOVAKIA, V5, P133 lshan Kailash, 2007, EUKARYOTIC CELL, V6, P1933 vka Adnan, 2011, BIOTECHNOLOGY AND BIOENGINEERING, V108, P2592 ye-Rowley WS, 2003, PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, VOL 73
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-142458DOI: 10.1186/s12934-017-0811-9ISI: 000415265500003PubMedID: 29137634OAI: oai:DiVA.org:umu-142458DiVA, id: diva2:1162305
Available from: 2017-12-04 Created: 2017-12-04 Last updated: 2017-12-04Bibliographically approved

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