Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
The neuronal and astrocytic protein SLC38A10 transports glutamine, glutamate, and aspartate, suggesting a role in neurotransmission
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Functional Pharmacology.ORCID iD: 0000-0002-6839-3651
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.ORCID iD: 0000-0002-9681-5129
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
2017 (English)In: FEBS Open Bio, E-ISSN 2211-5463, Vol. 7, no 6, p. 730-746Article in journal (Refereed) Published
Abstract [en]

In brain cells, glutamine transporters are vital to monitor and control the levels of glutamate and GABA. There are 11 members of the SLC38 family of amino acid transporters of which eight have been functionally characterized. Here, we report the first histological and functional characterization of the previously orphan member, SLC38A10. We used pairwise global sequence alignments to determine the sequence identity between the SLC38 family members. SLC38A10 was found to share 20-25% transmembrane sequence identity with several family members, and was predicted to have 11 transmembrane helices. SLC38A10 immunostaining was abundant in mouse brain using a custom-made anti-SLC38A10 antibody and colocalization of SLC38A10 immunoreactivity with markers for neurons and astrocytes was detected. Using Xenopus laevis oocytes overexpressing SLC38A10, we show that SLC38A10 mediates bidirectional transport of L-glutamine, L-alanine, L-glutamate, and D-aspartate, and efflux of L-serine. This profile mostly resembles system A members of the SLC38 family. In conclusion, the bidirectional transport of glutamine, glutamate, and aspartate by SLC38A10, and the immunostaining detected in neurons and astrocytes, suggest that SLC38A10 plays a role in pathways involved in neurotransmission.

Place, publisher, year, edition, pages
WILEY , 2017. Vol. 7, no 6, p. 730-746
Keyword [en]
glutamate/GABA-glutamine cycle, glutamine transport, immunohistochemistry, SLC38A10, solute carriers
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-330743DOI: 10.1002/2211-5463.12219ISI: 000404762100001PubMedID: 28593130OAI: oai:DiVA.org:uu-330743DiVA, id: diva2:1147544
Funder
Swedish Research CouncilThe Swedish Brain FoundationSwedish Society for Medical Research (SSMF)Novo NordiskStiftelsen Olle Engkvist ByggmästareMagnus Bergvall FoundationGunvor och Josef Anérs stiftelse
Available from: 2017-10-06 Created: 2017-10-06 Last updated: 2017-10-12Bibliographically approved

Open Access in DiVA

fulltext(1702 kB)20 downloads
File information
File name FULLTEXT01.pdfFile size 1702 kBChecksum SHA-512
4122d38e186224679fdd12087fe5434aad7962d3f89c62f32ade1bae49e36a1bde508ff32100417ff23cdfe2415cf57716d7eea586bc129ee7fe3f5c2a968cd4
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Hägglund, MariaEriksson, MikaelaFredriksson, Robert
By organisation
Department of Pharmaceutical BiosciencesFunctional Pharmacology
In the same journal
FEBS Open Bio
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 20 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 334 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf