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Mediator binds to boundaries of chromosomal interaction domains and to proteins involved in DNA looping, RNA metabolism, chromatin remodeling, and actin assembly
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
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2017 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 45, no 15, p. 8806-8821Article in journal (Refereed) Published
Abstract [en]

Mediator is a multi-unit molecular complex that plays a key role in transferring signals from transcriptional regulators to RNA polymerase II in eukaryotes. We have combined biochemical purification of the Sac-charomyces cerevisiae Mediator from chromatin with chromatin immunoprecipitation in order to reveal Mediator occupancy on DNA genome-wide, and to identify proteins interacting specifically with Mediator on the chromatin template. Tandem mass spectrometry of proteins in immunoprecipitates of mediator complexes revealed specific interactions between Mediator and the RSC, Arp2/Arp3, CPF, CF 1A and Lsm complexes in chromatin. These factors are primarily involved in chromatin remodeling, actin assembly, mRNA 3'-end processing, gene looping and mRNA decay, but they have also been shown to enter the nucleus and participate in Pol II transcription. Moreover, we have found that Mediator, in addition to binding Pol II promoters, occupies chromosomal interacting domain (CID) boundaries and that Mediator in chromatin associates with proteins that have been shown to interact with CID boundaries, such as Sth1, Ssu72 and histone H4. This suggests that Mediator plays a significant role in higher-order genome organization.

Place, publisher, year, edition, pages
Oxford University Press, 2017. Vol. 45, no 15, p. 8806-8821
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-139789DOI: 10.1093/nar/gkx491ISI: 000409380900020OAI: oai:DiVA.org:umu-139789DiVA, id: diva2:1146452
Available from: 2017-10-03 Created: 2017-10-03 Last updated: 2018-06-09Bibliographically approved

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Elfving, NilsBlomberg, JeanetteLarsson, MiriamBjörklund, Stefan
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