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The processive kinetics of gene conversion in bacteria
Harvard Univ, Dept Syst Biol, Boston, MA 02115 USA..
Harvard Univ, Dept Syst Biol, Boston, MA 02115 USA.;Univ Edinburgh, Inst Cell Biol, Sch Biol Sci, Edinburgh EH9 3JR, Midlothian, Scotland..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.ORCID iD: 0000-0002-7456-9182
2017 (English)In: Molecular Microbiology, ISSN 0950-382X, E-ISSN 1365-2958, Vol. 104, no 5, 752-760 p.Article in journal (Refereed) Published
Abstract [en]

Gene conversion, non-reciprocal transfer from one homologous sequence to another, is a major force in evolutionary dynamics, promoting co-evolution in gene families and maintaining similarities between repeated genes. However, the properties of the transfer - where it initiates, how far it proceeds and how the resulting conversion tracts are affected by mismatch repair - are not well understood. Here, we use the duplicate tuf genes in Salmonella as a quantitatively tractable model system for gene conversion. We selected for conversion in multiple different positions of tuf, and examined the resulting distributions of conversion tracts in mismatch repair-deficient and mismatch repair-proficient strains. A simple stochastic model accounting for the essential steps of conversion showed excellent agreement with the data for all selection points using the same value of the conversion processivity, which is the only kinetic parameter of the model. The analysis suggests that gene conversion effectively initiates uniformly at any position within a tuf gene, and proceeds with an effectively uniform conversion processivity in either direction limited by the bounds of the gene.

Place, publisher, year, edition, pages
John Wiley & Sons, 2017. Vol. 104, no 5, 752-760 p.
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-327241DOI: 10.1111/mmi.13661ISI: 000402864900005PubMedID: 28256783OAI: oai:DiVA.org:uu-327241DiVA: diva2:1130116
Available from: 2017-08-08 Created: 2017-08-08 Last updated: 2017-08-08Bibliographically approved

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