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Advanced Technologies for Detection of Cryptosporidum parvum in Drinking water: capture and detection using Microfluidic devices and Imaging Flow Cytometer
KTH, School of Architecture and the Built Environment (ABE), Land and Water Resources Engineering (moved 20130630).
2017 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Protecting drinking water supplies from pathogens such as Cryptosporidium parvum is a major concern for water treatment plants worldwide. The sensitivity and specificity of current detection methods are largely determined by the effectiveness of the concentration and separation methods used. In this study, disposable microfluidic micromixers were fabricated to effectively isolate Cryptosporidium parvum Oocysts from water samples, while allowing direct observation of Oocysts captured in the device using high quality immunofluorescence microscopy. In parallel, quantitative analysis of the capture yield was carried out by analyzing the waste from the microfluidics outlet with an Imaging Flow Cytometer. At the optimal flow rate, capture efficiencies higher than 95% were achieved in spiked samples, suggesting that scaled microfluidic isolation and detection of Cryptosporidium parvum will provide a faster and more efficient detection method for Cryptosporidium compared to other available laboratory-scale technologies.

Place, publisher, year, edition, pages
2017.
Series
TRITA-LWR Degree Project, ISSN 1651-064X ; 2017:06
Keyword [en]
Drinking water treatment, pathogen detection, Cryptosporidium, microfluidic devices, Imaging flow Cytometry, Capture efficiency
National Category
Civil Engineering
Identifiers
URN: urn:nbn:se:kth:diva-210729OAI: oai:DiVA.org:kth-210729DiVA: diva2:1119469
Educational program
Degree of Master - Water System Technology
Examiners
Available from: 2017-07-05 Created: 2017-07-04 Last updated: 2017-07-05Bibliographically approved

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fulltext(1271 kB)23 downloads
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f4b46838752fc755934213279d7442f2264eb05c8172bb28c9c53f424392af781a807a176868ea9d8c8d91d9a41c56ed0960ed8b3f9634432aba27601c810052
Type fulltextMimetype application/pdf

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Land and Water Resources Engineering (moved 20130630)
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CiteExportLink to record
Permanent link

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Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
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Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
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  • Other locale
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Output format
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