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(E)-α-bisabolene production in Synechocystis sp. PCC 6803
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Microbiology.
2017 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

The world is currently facing many environmental challenges related to fossil fuels and meanwhile the global energy demand is increasing rapidly. The terpenoid (E)-α-bisabolene is a precursor of the potential diesel fuel replacement bisabolane, and has been proven possible to produce in the cyanobacterial strain Synechocystis sp. PCC 6803. The aim of this project was to increase the bisabolene production in said strain, as well as introducing the same engineered system into Synechococcus elongatus PCC 7942 for comparison. For this purpose, several constructs were constructed which all contained the first enzyme of the terpenoid biosynthesis pathway; 1-Deoxy-D-xylulose 5-phosphate synthase (DXS). These constructs were then conjugated into the cyanobacterial strains after which the bisabolene production of positive colonies was evaluated. One of the constructs proved to increase the production of bisabolene in Synechocystis sp. PCC 6803, however the introduced DXS gene could not be detected neither on a genetic nor a translational level. Furthermore, the conjugation of Synechococcus elongatus PCC 7942 proved unsuccessful and did not give any colonies.

Place, publisher, year, edition, pages
2017.
Series
UPTEC X, 17 017
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-325811OAI: oai:DiVA.org:uu-325811DiVA: diva2:1116817
Educational program
Molecular Biotechnology Engineering Programme
Supervisors
Examiners
Available from: 2017-07-06 Created: 2017-06-27 Last updated: 2017-07-06Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
  • ieee
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