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The effects of thawing on the plasma metabolome: evaluating differences between thawed plasma and multi-organ samples
Umea Univ, Dept Chem, Computat Life Sci Cluster CLiC, Umea, Sweden.;Karlsruhe Inst Technol, Karlsruhe, Germany..
AcureOmics AB, Umea, Sweden..
AcureOmics AB, Umea, Sweden..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Environmental toxicology. AcureOmics AB, Umea, Sweden..
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2017 (English)In: Metabolomics, ISSN 1573-3882, E-ISSN 1573-3890, Vol. 13, no 6, 66Article in journal (Refereed) Published
Abstract [en]

Introduction Post-collection handling, storage and transportation can affect the quality of blood samples. Pre-analytical biases can easily be introduced and can jeopardize accurate profiling of the plasma metabolome. Consequently, a mouse study must be carefully planned in order to avoid any kind of bias that can be introduced, in order not to compromise the outcome of the study. The storage and shipment of the samples should be made in such a way that the freeze-thaw cycles are kept to a minimum. In order to keep the latent effects on the stability of the blood metabolome to a minimum it is essential to study the effect that the post-collection and pre-analytical error have on the metabolome.

Objectives The aim of this study was to investigate the effects of thawing on the metabolic profiles of different sample types.

Methods In the present study, a metabolomics approach was utilized to obtain a thawing profile of plasma samples obtained on three different days of experiment. The plasma samples were collected from the tail on day 1 and 3, while retro-orbital sampling was used on day 5. The samples were analysed using gas chromatography time-of-flight mass spectrometry (GC TOF-MS).

Results The thawed plasma samples were found to be characterized by higher levels of amino acids, fatty acids, glycerol metabolites and purine and pyrimidine metabolites as a result of protein degradation, cell degradation and increased phospholipase activity. The consensus profile was thereafter compared to the previously published study comparing thawing profiles of tissue samples from gut, kidney, liver, muscle and pancreas.

Conclusions The comparison between thawed organ samples and thawed plasma samples indicate that the organ samples are more sensitive to thawing, however thawing still affected all investigated sample types.

Place, publisher, year, edition, pages
2017. Vol. 13, no 6, 66
Keyword [en]
Mouse, Metabolomics, Plasma, Multi-organ, Freeze-thaw cycle, OPLS-DA
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-323768DOI: 10.1007/s11306-017-1196-9ISI: 000401711400001PubMedID: 28473743OAI: oai:DiVA.org:uu-323768DiVA: diva2:1107324
Funder
Swedish Research Council, 2011-6044EU, FP7, Seventh Framework Programme
Available from: 2017-06-09 Created: 2017-06-09 Last updated: 2017-06-09Bibliographically approved

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