Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
The regulatory effect of CsrA on cstA, glgCAP and flhDC in an RNase P ts mutant Escherichia coli
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre. (Leif Kirsebom Group)
2017 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

CsrA is a global post-transcriptional regulatory protein that has a great impact on many physiological pathways in a cell. It regulates central carbon metabolism, motility and biofilm formation as well as virulence, pathogenesis, quorum sensing and the oxidative stress response. However, CsrA is in turn also regulated by CsrB and CsrC sRNAs, among other factors. In this study, we explore its regulatory effect on three different genes/operons, cstA, glgCAP and flhDC. We performed beta-galactosidase assays on wild type (wt) and RNase P temperature sensitive (ts) cells to determine the dependence on RNaseP for the CsrA, CsrB and CsrC regulatory cascade on these three genes. Our results showed a clear decrease in cstA and glgCAP activity during CsrA activation, suggesting a regulatory cascade in which inhibition of RNase P leads to an inactivation of CsrB and CsrC. This in turn leads to an activation of CsrA and an inhibition of cstA and glgCAP. The effect on flhDC, however, was not as clear and needs further investigation.

Place, publisher, year, edition, pages
2017. , p. 19
Keywords [en]
CsrA, CsrB, CsrC, CsrD, GlgCAP, FlhDC, CstA, E. Coli, Glolbal regulator, RNase P, C5, ts, wt
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:uu:diva-322728OAI: oai:DiVA.org:uu-322728DiVA, id: diva2:1099165
Educational program
Bachelor Programme in Biology / Molecular Biology
Supervisors
Examiners
Available from: 2018-03-01 Created: 2017-05-29 Last updated: 2018-03-01Bibliographically approved

Open Access in DiVA

fulltext(704 kB)22 downloads
File information
File name FULLTEXT01.pdfFile size 704 kBChecksum SHA-512
4c255e01665fec43502a735d294d82f1afb5a23f82addfdbfbde481e775ee1e7895c93a51ea882951da520afcb56e2009db61cfd6c7d20525bd73cfe752830d4
Type fulltextMimetype application/pdf

By organisation
Biology Education Centre
Cell Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 22 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 86 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf