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Mixture-specific gene expression in zebrafish (Danio rerio) embryos exposed to perfluorooctane sulfonic acid (PFOS), perfluorohexanoic acid (PFHxA) and 3,3′,4,4′,5-pentachlorobiphenyl (PCB126)
Örebro University, School of Science and Technology, Örebro University, Sweden. (Man-Technology-Environment Research Centre, MTM)
Örebro University, School of Science and Technology, Örebro University, Sweden. (Man-Technology-Environment Research Centre, MTM)
Örebro University, School of Science and Technology, Örebro University, Sweden. (Man-Technology-Environment Research Centre, MTM)ORCID iD: 0000-0003-1733-8334
Örebro University, School of Science and Technology, Örebro University, Sweden. (Life Science)ORCID iD: 0000-0001-9713-2365
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2017 (English)In: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 590-591, 249-257 p.Article in journal (Refereed) Published
Abstract [en]

Perfluorooctane sulfonic acid (PFOS) and 3,3′,4,4′,5-pentachlorobiphenyl (PCB126) are persistent organic pollutants of high concern because of their environmental persistence, bioaccumulation and toxic properties. Besides, the amphiphilic properties of fluorinated compounds such as PFOS and perfluorohexanoic acid (PFHxA) suggest a role in increasing cell membrane permeability and solubilizing chemicals. The present study aimed at investigating whether PFOS and PFHxA are capable of modifying the activation of PCB126 toxicity-related pathways. For this purpose, zebrafish embryos were exposed in semi-static conditions to 7.5 μg/L of PCB126 alone, in the presence of 25 mg/L of PFOS, 15.7 mg/L of PFHxA or in the presence of both PFOS and PFHxA. Quantitative PCR was performed on embryos aged from 24 h post fertilization (hpf) to 96 hpf to investigate expression changes of genes involved in metabolism of xenobiotics (ahr2, cyp1a), oxidative stress (gpx1a, tp53), lipids metabolism (acaa2, osbpl1a), and epigenetic mechanisms (dnmt1, dnmt3ba). Cyp1a and ahr2 expression were significantly induced by the presence of PCB126. However, after 72 and 78 h of exposure, induction of cyp1a expression was significantly lower when embryos were co-exposed to PCB126 + PFOS + PFHxA when compared to PCB126-exposed embryos. Significant upregulation of gpx1a occurred after exposure to PCB126 + PFHxA and to PCB126 + PFOS + PFHxA at 30 and 48 hpf. Besides, embryos appeared more sensitive to PCB126 + PFOS + PFHxA at 78 hpf: acaa2 and osbpl1a were significantly downregulated; dnmt1 was significantly upregulated. While presented as environmentally safe, PFHxA demonstrated that it could affect gene expression patterns in zebrafish embryos when combined to PFOS and PCB126, suggesting that such mixture may increase PCB126 toxicity. This is of particular relevance since PFHxA is persistent and still being ejected into the environment. Moreover, it provides additional information as to the importance to integrate mixture effects of chemicals in risk assessment and biomonitoring frameworks.

Place, publisher, year, edition, pages
Elsevier, 2017. Vol. 590-591, 249-257 p.
Keyword [en]
Mixture toxicity PFAS Molecular pathway Zebrafish cyp1a Lipids
National Category
Environmental Sciences Biochemistry and Molecular Biology
Research subject
Enviromental Science
Identifiers
URN: urn:nbn:se:oru:diva-57792DOI: 10.1016/j.scitotenv.2017.02.232ISI: 000399511800026PubMedID: 28283292Scopus ID: 2-s2.0-85014558344OAI: oai:DiVA.org:oru-57792DiVA: diva2:1097302
Note

Funding agencies:

ERASMUS program 

Available from: 2017-05-22 Created: 2017-05-22 Last updated: 2017-05-24Bibliographically approved

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Blanc, MélanieKärrman, AnnaKukučka, PetrScherbak, NikolaiKeiter, Steffen
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