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Genome Dynamics of Escherichia coli during Antibiotic Treatment: Transfer, Loss, and Persistence of Genetic Elements In situ of the Infant Gut
Tech Univ Denmark, Novo Nordisk Fdn, Ctr Biosustainabil, Lyngby, Denmark..
Hvidovre Univ Hosp, Dept Clin Microbiol, Hvidovre, Denmark..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Univ Gothenburg, Sahlgrenska Acad, Dept Infect Dis, Gothenburg, Sweden..
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2017 (English)In: Frontiers in Cellular and Infection Microbiology, E-ISSN 2235-2988, Vol. 7, article id 126Article in journal (Refereed) Published
Abstract [en]

Elucidating the adaptive strategies and plasticity of bacterial genomes in situ is crucial for understanding the epidemiology and evolution of pathogens threatening human health. While much is known about the evolution of Escherichia coli in controlled laboratory environments, less effort has been made to elucidate the genome dynamics of E. coli in its native settings. Here, we follow the genome dynamics of co-existing E. coli lineages in situ of the infant gut during the first year of life. One E. coli lineage causes a urinary tract infection (UTI) and experiences several alterations of its genomic content during subsequent antibiotic treatment. Interestingly, all isolates of this uropathogenic E. coli strain carried a highly stable plasmid implicated in virulence of diverse pathogenic strains from all over the world. While virulence elements are certainly beneficial during infection scenarios, their role in gut colonization and pathogen persistence is poorly understood. We performed in vivo competitive fitness experiments to assess the role of this highly disseminated virulence plasmid in gut colonization, but found no evidence for a direct benefit of plasmid carriage. Through plasmid stability assays, we demonstrate that this plasmid is maintained in a parasitic manner, by strong first-line inheritance mechanisms, acting on the single-cell level, rather than providing a direct survival advantage in the gut. Investigating the ecology of endemic accessory genetic elements, in their pathogenic hosts and native environment, is of vital importance if we want to understand the evolution and persistence of highly virulent and drug resistant bacterial isolates.

Place, publisher, year, edition, pages
FRONTIERS MEDIA SA , 2017. Vol. 7, article id 126
Keywords [en]
Escherichia coli, genome evolution, virulence plasmid dynamics, plasmid persistence, horizontal gene transfer, antibiotic treatment, urinary tract infections, infant gut
National Category
Immunology in the medical area Microbiology in the medical area
Identifiers
URN: urn:nbn:se:uu:diva-322184DOI: 10.3389/fcimb.2017.00126ISI: 000399428400001PubMedID: 28447026OAI: oai:DiVA.org:uu-322184DiVA, id: diva2:1096092
Funder
EU, European Research Council, ERC-20104-STG LimitMDR (638902)Swedish Society for Medical Research (SSMF)Available from: 2017-05-17 Created: 2017-05-17 Last updated: 2018-01-13Bibliographically approved

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