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Giardia intestinalis mitosomes undergo synchronized fission but not fusion and are constitutively associated with the endoplasmic reticulum
Charles Univ Prague, Dept Parasitol, Fac Sci, Prumyslova 595, Vestec 25242, Czech Republic..
Charles Univ Prague, Dept Parasitol, Fac Sci, Prumyslova 595, Vestec 25242, Czech Republic..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Microbiology.
Charles Univ Prague, Inst Microbiol & Immunol, Fac Med 1, Prague, Czech Republic.;Gen Univ Hosp, Prague, Czech Republic..
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2017 (English)In: BMC Biology, ISSN 1741-7007, E-ISSN 1741-7007, Vol. 15, 27Article in journal (Refereed) Published
Abstract [en]

Background: Mitochondria of opisthokonts undergo permanent fission and fusion throughout the cell cycle. Here, we investigated the dynamics of the mitosomes, the simplest forms of mitochondria, in the anaerobic protist parasite Giardia intestinalis, a member of the Excavata supergroup of eukaryotes. The mitosomes have abandoned typical mitochondrial traits such as the mitochondrial genome and aerobic respiration and their single role known to date is the formation of iron-sulfur clusters. Results: In live experiments, no fusion events were observed between the mitosomes in G. intestinalis. Moreover, the organelles were highly prone to becoming heterogeneous. This suggests that fusion is either much less frequent or even absent in mitosome dynamics. Unlike in mitochondria, division of the mitosomes was absolutely synchronized and limited to mitosis. The association of the nuclear and the mitosomal division persisted during the encystation of the parasite. During the segregation of the divided mitosomes, the subset of the organelles between two G. intestinalis nuclei had a prominent role. Surprisingly, the sole dynamin- related protein of the parasite seemed not to be involved in mitosomal division. However, throughout the cell cycle, mitosomes associated with the endoplasmic reticulum (ER), although none of the known ER-tethering complexes was present. Instead, the ER-mitosome interface was occupied by the lipid metabolism enzyme long-chain acyl-CoA synthetase 4. Conclusions: This study provides the first report on the dynamics of mitosomes. We show that together with the loss of metabolic complexity of mitochondria, mitosomes of G. intestinalis have uniquely streamlined their dynamics by harmonizing their division with mitosis. We propose that this might be a strategy of G. intestinalis to maintain a stable number of organelles during cell propagation. The lack of mitosomal fusion may also be related to the secondary reduction of the organelles. However, as there are currently no reports on mitochondrial fusion in the whole Excavata supergroup, it is possible that the absence of mitochondrial fusion is an ancestral trait common to all excavates.

Place, publisher, year, edition, pages
2017. Vol. 15, 27
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Biological Sciences
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URN: urn:nbn:se:uu:diva-321187DOI: 10.1186/s12915-017-0361-yISI: 000398125900001PubMedID: 28372543OAI: oai:DiVA.org:uu-321187DiVA: diva2:1092337
Available from: 2017-05-02 Created: 2017-05-02 Last updated: 2017-05-02

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