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Functionalized capillaries for label- free bio-molecule-detection utilizing the streaming current method
KTH, School of Information and Communication Technology (ICT).
KTH, School of Information and Communication Technology (ICT).
2016 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

This report aims to make a contribution in optimizing a bio-sensor which exploits the changes in streaming current in a fused silica capillary as target molecules bind to immobilized receptors on the inner surface of the capillary. In this study we investigate the temporal characteristics of the sensor response as a function of capillary-dimension for different concentrations of target biomolecules. Using Heceptine-z domain interaction pairs and silica capillaries with different inner diameters in the range of 25-55 µm and lengthin the range of 3-9 cm we show that the reduction in capillary cross-section re-sults in a faster sensor response. The results show qualitative agreement withan analytical model grounded on diffusion-based mass transport under laminar flow condition and offer possibility to achieve a lower limit of detection with thinner capillaries.

Abstract [sv]

Denna rapport bidrar i arbetet att optimera en biosensor baserad på den flödes-inducerade elektriska strömmen som uppstår i en glaskapillär där målmolekyler i en lösning binder till immobiliserade receptorer på den inre ytan av kapillären. I experimenten som här presenteras har tidsberoendet hos sen-sorsvaret undersökts för olika koncentrationer av målmolekylen, från 10 nm till 1 nm. Interaktionsparet (Immuglobin 1G och antikropp)(Heceptine-z) användes i dessa experiment och med att testa olika glaskapillärer med innerdiametrar i intervallet 25 till 55 µm, samt längd mellan 3 och 9 cm, visar vi att en minskning av innerdiametern resulterar i snabbare sensorsvar. Resultaten visar påen kvalitativ överrenstämmelse med en analytisk modell grundad i diffusionsbaserad masstransport med krav på laminärt flöde. Denna modell tyder på att det är möjligt att detektera lägre koncentrationen med tunnare kapillärer än vad här har testats.

Place, publisher, year, edition, pages
2016. , 22 p.
Series
TRITA-ICT-EX, 2016:163
National Category
Electrical Engineering, Electronic Engineering, Information Engineering
Identifiers
URN: urn:nbn:se:kth:diva-206081OAI: oai:DiVA.org:kth-206081DiVA: diva2:1091124
Subject / course
Information and Communication Technology
Educational program
Master of Science in Engineering - Microelectronics
Supervisors
Examiners
Available from: 2017-04-26 Created: 2017-04-26 Last updated: 2017-04-27Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
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