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Evolution of group I introns in Porifera: new evidence for intron mobility and implications for DNA barcoding
Ludwig Maximilians Univ Munchen, Dept Earth & Environm Sci Palaeontol & Geobiol, Richard Wagner Str 10, D-80333 Munich, Germany..
Nova Southeastern Univ, Halmos Coll Nat Sci & Oceanog, Dania, FL 33004 USA..
Wageningen Univ & Res Ctr, Marine Anim Ecol, POB, NL-3700 AH Wageningen, Netherlands.;Naturalis Biodivers Ctr, Marine Zool Dept, POB 95172300RA, Leiden, Netherlands..
Natl Inst Water & Atmospher Res, Natl Ctr Aquat Biodivers & Biosecur, POB 109-695, Auckland, New Zealand..
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2017 (English)In: BMC Evolutionary Biology, ISSN 1471-2148, E-ISSN 1471-2148, Vol. 17, 82Article in journal (Refereed) Published
Abstract [en]

Background: Mitochondrial introns intermit coding regions of genes and feature characteristic secondary structures and splicing mechanisms. In metazoans, mitochondrial introns have only been detected in sponges, cnidarians, placozoans and one annelid species. Within demosponges, group I and group II introns are present in six families. Based on different insertion sites within the cox1 gene and secondary structures, four types of group I and two types of group II introns are known, which can harbor up to three encoding homing endonuclease genes (HEG) of the LAGLIDADG family (group I) and/or reverse transcriptase (group II). However, only little is known about sponge intron mobility, transmission, and origin due to the lack of a comprehensive dataset. We analyzed the largest dataset on sponge mitochondrial group I introns to date: 95 specimens, from 11 different sponge genera which provided novel insights into the evolution of group I introns. Results: For the first time group I introns were detected in four genera of the sponge family Scleritodermidae (Scleritoderma, Microscleroderma, Aciculites, Setidium). We demonstrated that group I introns in sponges aggregate in the most conserved regions of cox1. We showed that co-occurrence of two introns in cox1 is unique among metazoans, but not uncommon in sponges. However, this combination always associates an active intron with a degenerating one. Earlier hypotheses of HGT were confirmed and for the first time VGT and secondary losses of introns conclusively demonstrated. Conclusion: This study validates the subclass Spirophorina (Tetractinellida) as an intron hotspot in sponges. Our analyses confirm that most sponge group I introns probably originated from fungi. DNA barcoding is discussed and the application of alternative primers suggested.

Place, publisher, year, edition, pages
BIOMED CENTRAL LTD , 2017. Vol. 17, 82
Keyword [en]
Porifera, Tetractinellida, cox1, HGT, VGT, homing endonuclease gene (HEG), LAGLIDADG, group I intron, DNA barcoding
National Category
Evolutionary Biology Genetics
Identifiers
URN: urn:nbn:se:uu:diva-320206DOI: 10.1186/s12862-017-0928-9ISI: 000397338000001PubMedID: 28320321OAI: oai:DiVA.org:uu-320206DiVA: diva2:1088971
Funder
German Research Foundation (DFG), DFG ER 611/3-1 DFG Wo896/15-1
Available from: 2017-04-18 Created: 2017-04-18 Last updated: 2017-04-18Bibliographically approved

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