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Detection and localization of viral infection in the pancreas of patients with type 1 diabetes using short fluorescently-labelled oligonucleotide probes
Univ Bremen, Islet Biol Lab, Bremen, Germany..
Univ Bremen, Islet Biol Lab, Bremen, Germany..
Univ Exeter, Islet Biol Exeter, Med, Exeter EX4 4QJ, Devon, England..
Univ Tampere, Sch Med, Dept Virol, Tampere, Finland..
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2017 (English)In: OncoTarget, ISSN 1949-2553, E-ISSN 1949-2553, Vol. 8, no 8, p. 12620-12636Article in journal (Refereed) Published
Abstract [en]

Enteroviruses, specifically of the Coxsackie B virus family, have been implicated in triggering islet autoimmunity and type 1 diabetes, but their presence in pancreata of patients with diabetes has not been fully confirmed. To detect the presence of very low copies of the virus genome in tissue samples from T1D patients, we designed a panel of fluorescently labeled oligonucleotide probes, each of 17-22 nucleotides in length with a unique sequence to specifically bind to the enteroviral genome of the picornaviridae family. With these probes enteroviral RNA was detected with high sensitivity and specificity in infected cells and tissues, including in FFPE pancreas sections from patients with T1D. Detection was not impeded by variations in sample processing and storage thereby overcoming the potential limitations of fragmented RNA. Co-staining of small RNA probes in parallel with classical immunstaining enabled virus detection in a cell-specific manner and more sensitively than by viral protein.

Place, publisher, year, edition, pages
IMPACT JOURNALS LLC , 2017. Vol. 8, no 8, p. 12620-12636
Keywords [en]
enteroviruses, type 1 diabetes, pancreas, islets, oligonucleotide probes, Pathology Section
National Category
Cancer and Oncology Cell Biology
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URN: urn:nbn:se:uu:diva-319301DOI: 10.18632/oncotarget.14896ISI: 000395692000017OAI: oai:DiVA.org:uu-319301DiVA, id: diva2:1087960
Available from: 2017-04-10 Created: 2017-04-10 Last updated: 2017-11-29Bibliographically approved

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