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The adenovirus L4-22K protein regulates transcription and RNA splicing via a sequence-specific single-stranded RNA binding
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
2017 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 45, no 4, p. 1731-1742Article in journal (Refereed) Published
Abstract [en]

The adenovirus L4-22K protein both activates and suppresses transcription from the adenovirus major late promoter (MLP) by binding to DNA elements located downstream of the MLP transcriptional start site: the so-called DE element ( positive) and the R1 region ( negative). Here we show that L4-22K preferentially binds to the RNA form of the R1 region, both to the double-stranded RNA and the single-stranded RNA of the same polarity as the nascent MLP transcript. Further, L4-22K binds to a 5'-CAAA-3' motif in the single-stranded RNA, which is identical to the sequence motif characterized for L4-22K DNA binding. L4-22K binding to single-stranded RNA results in an enhancement of U1 snRNA recruitment to the major late first leader 5' splice site. This increase in U1 snRNA binding results in a suppression of MLP transcription and a concurrent stimulation of major late first intron splicing.

Place, publisher, year, edition, pages
2017. Vol. 45, no 4, p. 1731-1742
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-318946DOI: 10.1093/nar/gkw1145ISI: 000396055400023PubMedID: 27899607OAI: oai:DiVA.org:uu-318946DiVA, id: diva2:1086540
Funder
Swedish Cancer Society, 120678, 130410Swedish Research Council, 2006-5038-36531-16Available from: 2017-04-03 Created: 2017-04-03 Last updated: 2017-11-29Bibliographically approved

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Lan, SusanKamel, WaelPunga, TanelAkusjärvi, Göran
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