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Mucin preparation and assembly into new biomaterials
KTH, School of Technology and Health (STH), Medical Engineering.
2016 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Mucins, the main macromolecular constituent responsible for gel-forming property in mucus, have great potential to act as new biological hydrogel for medical applications. Click chemistry reaction is an attractive tool to be applied in both bioconjugation and material science to form covalent bonds between molecules. Herein the click chemistry reaction of tetrazine-norbornene ligation was adapted to form click mucin hydrogel using purified commercial available bovine submaxillary mucin (BSM). This study included the characterization, purification and chemical modification of commercial available BSM. The flow filtration purification was chosen after investigating the effectiveness and yields of four different purification strategies. The reactivity of tetrazine and norbornene-functionalized BSM was evident from the formation of robust mucin hydrogel within minutes after mixing the two components. 

Abstract [sv]

Mucin, den viktigaste makromolekylära beståndsdel som ansvarar för den gelbildande egenskapen i slem, har stor potential att fungera som en ny biologisk hydrogel för medicinska tillämpningar. Klick-kemi reaktioner är attraktiva verktyg som kan användas i både biokonjugering och materialvetenskap för att bilda kovalenta bindningar mellan molekyler. I detta projekt användes renat kommersiellt köpt bovint submaxillärt mucin (BSM) i en klick-kemi reaktion för att sammanlänka tetrazin och norbornylen. Denna reaktion anpassades för att bilda en mucin hydrogel. Detta projekt inkluderade karakterisering, rening och kemisk modifiering av kommersiellt köpt BSM. Flödesfiltrering valdes som reningsmetod efter undersöking av effektivitet och utbyte av fyra olika reningsstrategier. Reaktiviteten hos tetrazin och norbornen-funktionaliserad BSM var uppenbar från bildandet av robust mucin hydrogel inom några minuter efter de två komponenterna sammanblandats. 

Place, publisher, year, edition, pages
2016. , 41 p.
Series
TRITA-STH, 2016:87
Keyword [en]
BSM􏰀, characterization􏰀, purification, 􏰀 chemical modification􏰀, hydrogel
Keyword [sv]
BSM, karakterisering, rening, kemisk modifiering, hydrogel
National Category
Medical Engineering
Identifiers
URN: urn:nbn:se:kth:diva-204843OAI: oai:DiVA.org:kth-204843DiVA: diva2:1086182
Subject / course
Medical Engineering
Educational program
Master of Science - Medical Engineering
Presentation
2016-06-10, 3-221, ANA 10, Alfred Nobels alle 10, 14152 Huddinge, Huddinge, 11:00 (English)
Supervisors
Examiners
Available from: 2017-04-27 Created: 2017-03-31 Last updated: 2017-04-27Bibliographically approved

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