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Three new antisera with high sensitivity to ecdysone, 3-dehydroecdysone and other A-ring derivatives: Production and characterization
Institut für Zoophysiologie, Universität Bonn, Germany.
Université de Bourgogne, Laboratoire de Zoologie, France.
Ecole Normale Supérieure, Laboratoire de Biochimie et Physiologie du Développement, France.
Institut für Zoophysiologie, Universität Bonn, Germany.
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1995 (English)In: European Journal of Entomology, ISSN 1210-5759, E-ISSN 1802-8829, Vol. 92, no 1, p. 75-79Article in journal (Refereed) Published
Abstract [en]

Three new antisera have been produced in rabbits immunized with E-2/3-hemisuccinate conjugates. In a competitive enzyme immunoassay (EIA) with 20-ecdysone-peroxidase as a tracer, the antisera exhibit high specificity towards the side chain of the ecdysone (E) molecule. Modifications of the A-ring do result in no or small changes of sensitivity. One antiserum (RUD-2) allows the direct quantification of total ecdysteroids in samples containing E and 3-dehydroecdysone in varying ratios, without further sophisticated sample treatment. At least one of the other antisera allows the direct quantification of mixtures of E and 25-deoxyecdysone (RUD-1). Thus, these antisera may be a useful tool in studying molting control in arthropods.

Place, publisher, year, edition, pages
1995. Vol. 92, no 1, p. 75-79
Keywords [en]
Antisera, ecdysone, ecdysteroid, EIA, enzyme immunoassay, molt
National Category
Zoology
Research subject
Zoology
Identifiers
URN: urn:nbn:se:su:diva-140898OAI: oai:DiVA.org:su-140898DiVA, id: diva2:1083617
Available from: 2017-03-21 Created: 2017-03-21 Last updated: 2017-11-29Bibliographically approved

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