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The Chd1 chromatin remodeler shifts hexasomes unidirectionally
Johns Hopkins Univ, TC Jenkins Dept Biophys, Baltimore, MD 21218 USA..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Systems Biology. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology. Uppsala University, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0001-6807-8654
Johns Hopkins Univ, TC Jenkins Dept Biophys, Baltimore, MD 21218 USA..
2016 (English)In: eLIFE, E-ISSN 2050-084X, Vol. 5, article id e21356Article in journal (Refereed) Published
Abstract [en]

Despite their canonical two-fold symmetry, nucleosomes in biological contexts are often asymmetric: functionalized with post-translational modifications (PTMs), substituted with histone variants, and even lacking H2A/H2B dimers. Here we show that the Widom 601 nucleosome positioning sequence can produce hexasomes in a specific orientation on DNA, providing a useful tool for interrogating chromatin enzymes and allowing for the generation of nucleosomes with precisely defined asymmetry. Using this methodology, we demonstrate that the Chd1 chromatin remodeler from Saccharomyces cerevisiae requires H2A/H2B on the entry side for sliding, and thus, unlike the back-and-forth sliding observed for nucleosomes, Chd1 shifts hexasomes unidirectionally. Chd1 takes part in chromatin reorganization surrounding transcribing RNA polymerase II (Pol II), and using asymmetric nucleosomes we show that ubiquitin-conjugated H2B on the entry side stimulates nucleosome sliding by Chd1. We speculate that biased nucleosome and hexasome sliding due to asymmetry contributes to the packing of arrays observed in vivo.

Place, publisher, year, edition, pages
2016. Vol. 5, article id e21356
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-316966DOI: 10.7554/eLife.21356ISI: 000393418200001PubMedID: 28032848OAI: oai:DiVA.org:uu-316966DiVA, id: diva2:1079501
Funder
NIH (National Institute of Health), R01-GM084192 T32-GM007231Swedish Research CouncilKnut and Alice Wallenberg Foundation
Available from: 2017-03-08 Created: 2017-03-08 Last updated: 2018-01-13Bibliographically approved

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