Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Humanin skeletal muscle protein levels increase after resistance training in men with impaired glucose metabolism
Karolinska Inst, Dept Physiol & Pharmacol, Von Eulers Vag 8, S-17177 Stockholm, Sweden..
Karolinska Inst, Dept Womens & Childrens Hlth, Neuropediat Unit, Stockholm, Sweden.;Astrid Lindgrens Pediat Hosp, Stockholm, Sweden..
Univ Eastern Finland, Inst Biomed Sports & Exercise Med, Kuopio, Finland..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences, Clinical Nutrition and Metabolism.ORCID iD: 0000-0002-8620-4586
Show others and affiliations
2016 (English)In: Physiological Reports, E-ISSN 2051-817X, Vol. 4, no 23, article id e13063Article in journal (Refereed) Published
Abstract [en]

Humanin (HN) is a mitochondrially encoded and secreted peptide linked to glucose metabolism and tissue protecting mechanisms. Whether skeletal muscle HN gene or protein expression is influenced by exercise remains unknown. In this intervention study we show, for the first time, that HN protein levels increase in human skeletal muscle following 12 weeks of resistance training in persons with prediabetes. Male subjects (n = 55) with impaired glucose regulation (IGR) were recruited and randomly assigned to resistance training, Nordic walking or a control group. The exercise interventions were performed three times per week for 12 weeks with progressively increased intensity during the intervention period. Biopsies from the vastus lateralis muscle and venous blood samples were taken before and after the intervention. Skeletal muscle and serum protein levels of HN were analyzed as well as skeletal muscle gene expression of the mitochondrially encoded gene MT-RNR2, containing the open reading frame for HN. To elucidate mitochondrial training adaptation, mtDNA, and nuclear DNA as well as Citrate synthase were measured. Skeletal muscle HN protein levels increased by 35% after 12 weeks of resistance training. No change in humanin protein levels was seen in serum in any of the intervention groups. There was a significant correlation between humanin levels in serum and the improvements in the 2 h glucose loading test in the resistance training group. The increase in HN protein levels in skeletal muscle after regular resistance training in prediabetic males may suggest a role for HN in the regulation of glucose metabolism. Given the preventative effect of exercise on diabetes type 2, the role of HN as a mitochondrially derived peptide and an exercise-responsive mitokine warrants further investigation.

Place, publisher, year, edition, pages
2016. Vol. 4, no 23, article id e13063
Keyword [en]
Exercise, human, humanin, impaired glucose regulation, skeletal muscle
National Category
Physiology
Identifiers
URN: urn:nbn:se:uu:diva-316145DOI: 10.14814/phy2.13063ISI: 000392242400009OAI: oai:DiVA.org:uu-316145DiVA, id: diva2:1077474
Available from: 2017-02-27 Created: 2017-02-27 Last updated: 2018-01-13Bibliographically approved

Open Access in DiVA

fulltext(190 kB)91 downloads
File information
File name FULLTEXT01.pdfFile size 190 kBChecksum SHA-512
e27124a10e9ea12f5f4a17a01036904972f84a568482c3c5d4e1023ffaf8fb9b17fc6c41ddf6b8ed5abb0e236d7b785c0eb84dc6b9295e3c34771a50b151c8b1
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Risérus, Ulf
By organisation
Clinical Nutrition and Metabolism
In the same journal
Physiological Reports
Physiology

Search outside of DiVA

GoogleGoogle Scholar
Total: 91 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 468 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf