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Sex differences and effects of oestrogen in rat gastric mucosal defence
Karolinska Inst, Dept Clin Sci Intervent & Technol CLINTEC, S-14186 Stockholm, Sweden.;Karolinska Univ Hosp, Funct Perioperat Med & Intens Care, S-17176 Stockholm, Sweden..
Karolinska Univ Hosp, Funct Perioperat Med & Intens Care, S-17176 Stockholm, Sweden.;Karolinska Inst, Dept Physiol & Pharmacol, S-17177 Stockholm, Sweden..
Kyoto Prefectural Univ Med, Grad Sch Med Sci, Dept Endocrine & Breast Surg, Kamigyo Ku, 465 Kajii Cho, Kyoto 6020841, Japan..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology, Integrative Physiology.
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2017 (English)In: World Journal of Gastroenterology, ISSN 1007-9327, E-ISSN 2219-2840, Vol. 23, no 3, p. 426-436Article in journal (Refereed) Published
Abstract [en]

AIM To evaluate sex differences and the effects of oestrogen administration in rat gastric mucosal defence. METHODS Sex differences in gastric mucus thickness and accumulation rate, absolute gastric mucosal blood flow using microspheres, the integrity of the gastric mucosal epithelium in response to a chemical irritant and the effects of oestrogen administration on relative gastric mucosal blood flow in an acute setting was assessed in an in vivo rat experimental model. Subsequently, sex differences in the distribution of oestrogen receptors and calcitonin gene related peptide in the gastric mucosa of animals exposed to oestrogen in the above experiments was evaluated using immunohistochemistry. RESULTS The absolute blood flow in the GI-tract was generally higher in males, but only significantly different in the corpus part of the stomach (1.12 +/- 0.12 mL/min.g in males and 0.51 +/- 0.03 mL/min.g in females) (P = 0.002). After removal of the loosely adherent mucus layer the thickness of the firmly adherent mucus layer in males and females was 79 +/- 1 mu m and 80 +/- 3 mu m respectively. After 60 min the mucus thickness increased to 113 +/- 3 mu m in males and 121 +/- 3 mu m in females with no statistically significant difference seen between the sexes. Following oestrogen administration (0.1 followed by 1 mu g/kg.min), mean blood flow in the gastric mucosa decreased by 31% [68 +/- 13 perfusion units (PFU)] in males which was significantly different compared to baseline (P = 0.02). In females however, mean blood flow remained largely unchanged with a 4% (5 +/- 33 PFU) reduction. The permeability of the gastric mucosa increased to a higher level in females than in males (P = 0.01) after taurocholate challenge. However, the calculated mean clearance increase did not significantly differ between the sexes [0.1 +/- 0.04 to 1.1 +/- 0.1 mL/min.100 g in males and 0.4 +/- 0.3 to 2.1 +/- 0.3 mL/min.100 g in females (P = 0.065)]. There were no significant differences between 17 beta-Estradiol treated males (mean ratio of positive staining +/- SEM) (0.06 +/- 0.07) and females (0.11 +/- 0.11) in the staining of ER alpha (P = 0.24). Also, there were no significant differences between 17 beta-Estradiol treated males (0.18 +/- 0.21) and females (0.06 +/- 0.12) in the staining of ER beta (P = 0.11). Finally, there were no significant differences between 17 beta-Estradiol treated males (0.04 +/- 0.05) and females (0.11 +/- 0.10) in the staining of CGRP (P = 0.14). CONCLUSION Gastric mucosal blood flow is higher in male than in female rats and is reduced in male rats by oestrogen administration.

Place, publisher, year, edition, pages
2017. Vol. 23, no 3, p. 426-436
Keywords [en]
Sex differences, Gastric mucosal defence, Blood flow, Oestrogen, Gastric physiology, Mucus
National Category
Gastroenterology and Hepatology
Identifiers
URN: urn:nbn:se:uu:diva-316024DOI: 10.3748/wjg.v23.i3.426ISI: 000391956000005OAI: oai:DiVA.org:uu-316024DiVA, id: diva2:1076934
Available from: 2017-02-24 Created: 2017-02-24 Last updated: 2017-11-29Bibliographically approved

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