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Spatholobus suberectus Column Extract Inhibits Estrogen Receptor Positive Breast Cancer via Suppressing ER MAPK PI3K/AKT Pathway
Capital Med Univ, Beijing Hosp Tradit Chinese Med, Dept Oncol, Beijing, Peoples R China..
Capital Med Univ, Beijing Hosp Tradit Chinese Med, Dept Oncol, Beijing, Peoples R China..
Capital Med Univ, Beijing Hosp Tradit Chinese Med, Dept Oncol, Beijing, Peoples R China..
Capital Med Univ, Beijing Hosp Tradit Chinese Med, Dept Oncol, Beijing, Peoples R China..
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2016 (English)In: Evidence-based Complementary and Alternative Medicine, ISSN 1741-427X, E-ISSN 1741-4288, article id 2934340Article in journal (Refereed) Published
Abstract [en]

Although Chinese herbal compounds have long been alternatively applied for cancer treatment in China, their treatment effects have not been sufficiently investigated. The Chinese herb Spatholobus suberectus is commonly prescribed to cancer patients. HPLC analysis has shown that the main components of Spatholobus suberectus are flavonoids that can be classified as phytoestrogens, having a structure similar to estrogen. This study was designed to investigate the effects of Spatholobus suberectus column extract (SSCE) on the estrogen receptor-positive (ER+) breast cancer cell line MCF-7 and its possible molecular mechanism. In our study, MTT assay was performed to evaluate cell viability. The results show that SSCE (80, 160, and 320 mu g/ml) significantly decreased the viability of MCF-7 cells. SSCE also triggered apoptosis, arrested the cell cycle at the G0/G1 phase, and inhibited cell migration. A dual-luciferase reporter system showed that SSCE suppressed intranuclear p-ER activity; Western blot analysis confirmed the repressed expression of phosphorylated-ER alpha (p-ER alpha), ERK1/2, p-ERK1/2, AKT, p-AKT, p-mTOR, PI3K, and p-PI3K, indicating that SSCE suppressed the MAPK PI3K/AKT signaling pathway. Collectively, our results suggest that SSCE causes apoptosis, an arrest in the G0/G1 phase, and a decrease in migration in ER+ MCF-7 cells via hypoactivity of the ER and suppression of the MAPK PI3K/AKT pathway.

Place, publisher, year, edition, pages
2016. article id 2934340
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Cell and Molecular Biology
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URN: urn:nbn:se:uu:diva-315102DOI: 10.1155/2016/2934340ISI: 000391608800001OAI: oai:DiVA.org:uu-315102DiVA, id: diva2:1072767
Available from: 2017-02-08 Created: 2017-02-08 Last updated: 2018-01-13Bibliographically approved

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