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A Linkage Map and QTL Analysis for Pyrethroid Resistance in the Bed Bug Cimex lectularius
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Evolutionary Biology. Univ Helsinki, Dept Biosci, Helsinki, Finland.;Univ Sheffield, Dept Anim & Plant Sci, Sheffield, S Yorkshire, England..
Natl Inst Genet, Ecol Genet Div, Mishima, Shizuoka, Japan..
Univ Sheffield, Dept Anim & Plant Sci, Sheffield S10 2TN, S Yorkshire, England..
Tech Univ Dresden, Appl Zool, Dept Biol, D-01069 Dresden, Germany..
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2016 (English)In: G3: Genes, Genomes, Genetics, ISSN 2160-1836, E-ISSN 2160-1836, Vol. 6, no 12, p. 4059-4066Article in journal (Refereed) Published
Abstract [en]

The rapid evolution of insecticide resistance remains one of the biggest challenges in the control of medically and economically important pests. Insects have evolved a diverse range of mechanisms to reduce the efficacy of the commonly used classes of insecticides, and finding the genetic basis of resistance is a major aid to management. In a previously unstudied population, we performed an F-2 resistance mapping cross for the common bed bug, Cimex lectularius, for which insecticide resistance is increasingly widespread. Using 334 SNP markers obtained through RAD-sequencing, we constructed the first linkage map for the species, consisting of 14 putative linkage groups (LG), with a length of 407 cM and an average marker spacing of 1.3 cM. The linkage map was used to reassemble the recently published reference genome, facilitating refinement and validation of the current genome assembly. We detected a major QTL on LG12 associated with insecticide resistance, occurring in close proximity (1.2 Mb) to a carboxylesterase encoding candidate gene for pyrethroid resistance. This provides another example of this candidate gene playing a major role in determining survival in a bed bug population following pesticide resistance evolution. The recent availability of the bed bug genome, complete with a full list of potential candidate genes related to insecticide resistance, in addition to the linkage map generated here, provides an excellent resource for future research on the development and spread of insecticide resistance in this resurging pest species.

Place, publisher, year, edition, pages
2016. Vol. 6, no 12, p. 4059-4066
Keyword [en]
ectoparasite, household pest, insecticide, RAD-seq
National Category
Genetics and Breeding Genetics
Identifiers
URN: urn:nbn:se:uu:diva-313970DOI: 10.1534/g3.116.033092ISI: 000390591400025PubMedID: 27733453OAI: oai:DiVA.org:uu-313970DiVA, id: diva2:1072702
Funder
NERC - the Natural Environment Research Council
Note

Toby Fountain and Mark Ravinet contributed equally to this work.

Available from: 2017-02-08 Created: 2017-01-25 Last updated: 2017-11-29Bibliographically approved

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