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Integrating mass spectrometry with MD simulations reveals the role of lipids in Na+/H+ antiporters
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Biochemistry. (Erik Marklund)ORCID iD: 0000-0002-9804-5009
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2017 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 8, article id 13993Article in journal (Refereed) Published
Abstract [en]

Na+/H+ antiporters are found in all kingdoms of life and exhibit catalysis rates that are among the fastest of all known secondary-active transporters. Here we combine ion mobility mass spectrometry and molecular dynamics simulations to study the conformational stability and lipid-binding properties of the Na+/H+ exchanger NapA from Thermus thermophilus and compare this to the prototypical antiporter NhaA from Escherichia coli and the human homologue NHA2. We find that NapA and NHA2, but not NhaA, form stable dimers and do not selectively retain membrane lipids. By comparing wild-type NapA with engineered variants, we show that the unfolding of the protein in the gas phase involves the disruption of inter-domain contacts. Lipids around the domain interface protect the native fold in the gas phase by mediating contacts between the mobile protein segments. We speculate that elevator-type antiporters such as NapA, and likely NHA2, use a subset of annular lipids as structural support to facilitate large-scale conformational changes within the membrane.

Place, publisher, year, edition, pages
2017. Vol. 8, article id 13993
National Category
Structural Biology Bioinformatics (Computational Biology) Biophysics
Research subject
Biology with specialization in Structural Biology
Identifiers
URN: urn:nbn:se:uu:diva-314649DOI: 10.1038/ncomms13993ISI: 000391641800001PubMedID: 28071645OAI: oai:DiVA.org:uu-314649DiVA, id: diva2:1071341
Funder
Swedish Research Council, 2015-00559EU, FP7, Seventh Framework Programme, 2015-00559
Note

Authors Marklund and Uzdavinys contributed equally to this work.

Available from: 2017-02-03 Created: 2017-02-03 Last updated: 2018-01-13Bibliographically approved

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