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Introducing quality assessment and efficient management of cellular thermal shift assay mass spectrometry data
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2017 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Recent advances in molecular biology has led to the discovery of many new potential drugs. However, difficulties with in situ analysis of ligand binding prevents quick advancement in clinical trials, which stresses the need for better direct methods. A relatively new methodology, called Cellular Thermal Shift Assay (CETSA), allows for detection of ligand binding in a cells natural environment and can be used in combination with Mass Spectrometry (MS) for readout. With help from the Pelago Bioscience team, I developed a pipeline for processing of CETSA MS data and a web based system for viewing the results. The system, called CETSA Analytics, also evaluates the results relevance and helps its users to locate information efficiently. CETSA Analytics is currently being tested by Pelago Bioscience AB as a tool for experimental data distribution. 

Place, publisher, year, edition, pages
2017. , p. 35
Series
UPTEC X ; 16031
Keywords [en]
Cellular thermal shift assay, Information management system, Quality Assessent, Melt curve, Target engagement, Mass spectrometry
National Category
Information Systems Bioinformatics and Systems Biology Engineering and Technology
Identifiers
URN: urn:nbn:se:uu:diva-311792OAI: oai:DiVA.org:uu-311792DiVA, id: diva2:1061451
Educational program
Molecular Biotechnology Engineering Programme
Supervisors
Examiners
Available from: 2017-01-23 Created: 2017-01-02 Last updated: 2018-01-13Bibliographically approved

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Biology Education Centre
Information SystemsBioinformatics and Systems BiologyEngineering and Technology

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CiteExportLink to record
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  • apa
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