Change search
ReferencesLink to record
Permanent link

Direct link
Gene-specific correlation of RNA and protein levels in human cells and tissues
KTH Royal Inst Technol, Sci Life Lab, Stockholm, Sweden..
KTH Royal Inst Technol, Sci Life Lab, Stockholm, Sweden..
KTH Royal Inst Technol, Sci Life Lab, Stockholm, Sweden..
KTH Royal Inst Technol, Sci Life Lab, Stockholm, Sweden..
Show others and affiliations
2016 (English)In: Molecular Systems Biology, ISSN 1744-4292, E-ISSN 1744-4292, Vol. 12, no 10, 883Article in journal (Refereed) Published
Abstract [en]

An important issue for molecular biology is to establish whether transcript levels of a given gene can be used as proxies for the corresponding protein levels. Here, we have developed a targeted proteomics approach for a set of human non-secreted proteins based on parallel reaction monitoring to measure, at steady-state conditions, absolute protein copy numbers across human tissues and cell lines and compared these levels with the corresponding mRNA levels using transcriptomics. The study shows that the transcript and protein levels do not correlate well unless a gene-specific RNA-to-protein (RTP) conversion factor independent of the tissue type is introduced, thus significantly enhancing the predictability of protein copy numbers from RNA levels. The results show that the RTP ratio varies significantly with a few hundred copies per mRNA molecule for some genes to several hundred thousands of protein copies per mRNA molecule for others. In conclusion, our data suggest that transcriptome analysis can be used as a tool to predict the protein copy numbers per cell, thus forming an attractive link between the field of genomics and proteomics.

Place, publisher, year, edition, pages
2016. Vol. 12, no 10, 883
Keyword [en]
gene expression, protein quantification, targeted proteomics, transcriptomics
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-308938DOI: 10.15252/msb.20167144ISI: 000386948100001OAI: oai:DiVA.org:uu-308938DiVA: diva2:1051310
Funder
Knut and Alice Wallenberg Foundation
Available from: 2016-12-01 Created: 2016-12-01 Last updated: 2016-12-01Bibliographically approved

Open Access in DiVA

fulltext(1389 kB)15 downloads
File information
File name FULLTEXT01.pdfFile size 1389 kBChecksum SHA-512
b0f990af7eb0aace29fcc2cef7656ddbda31ffb46e8aa8d1c831511944255dfa568cae2f020b3f195182ce5b52e40fe1555a0411e9a0edea4c4016b0046ea342
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Pontén, Fredrik
By organisation
Clinical and experimental pathology
In the same journal
Molecular Systems Biology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 15 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 24 hits
ReferencesLink to record
Permanent link

Direct link