Change search
ReferencesLink to record
Permanent link

Direct link
Cell-specific nitrogen- and carbon-fixation of cyanobacteria in a temperate marine system (Baltic Sea).
Show others and affiliations
2016 (English)In: Environmental Microbiology Reports, ISSN 1758-2229, E-ISSN 1758-2229Article in journal (Refereed) Published
Abstract [en]

We analysed N2- and carbon (C) fixation in individual cells of Baltic Sea cyanobacteria by combining stable isotope incubations with secondary ion mass spectrometry (SIMS). Specific growth rates based on N2- and C-fixation were higher for cells of Dolichospermum spp. than for Aphanizomenon sp. and Nodularia spumigena. The cyanobacterial biomass, however, was dominated by Aphanizomenon sp., which contributed most to total N2-fixation in surface waters of the Northern Baltic Proper. N2-fixation by Pseudanabaena sp. and colonial picocyanobacteria was not detectable. N2-fixation by Aphanizomenon sp., Dolichospermum spp. and N. spumigena populations summed up to total N2-fixation, thus these genera appeared as sole diazotrophs within the Baltic Sea's euphotic zone, while their mean contribution to total C-fixation was 21%. Intriguingly, cell-specific N2-fixation was eightfold higher at a coastal station compared to an offshore station, revealing coastal zones as habitats with substantial N2-fixation. At the coastal station, the cell-specific C- to N2-fixation ratio was below the cellular C:N ratio, i.e. N2 was assimilated in excess to C-fixation, whereas the C- to N2-fixation ratio exceeded the C:N ratio in offshore sampled diazotrophs. Our findings highlight SIMS as a powerful tool not only for qualitative but also for quantitative N2-fixation assays in aquatic environments.

Place, publisher, year, edition, pages
2016.
National Category
Microbiology
Research subject
Diversity of life
Identifiers
URN: urn:nbn:se:nrm:diva-1952DOI: 10.1111/1462-2920.13557OAI: oai:DiVA.org:nrm-1952DiVA: diva2:1050926
Funder
Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning, 215-2009-813
Available from: 2016-11-30 Created: 2016-11-30 Last updated: 2016-11-30Bibliographically approved

Open Access in DiVA

fulltext(1459 kB)4 downloads
File information
File name FULLTEXT01.pdfFile size 1459 kBChecksum SHA-512
a5799215138cababf19f0b22995e91679a42fbe79eff5185a0ea87df9c6c3811e078ab85ffea1d361d49d5f29e1d22098f6e0bc85ce4c3c4bc82c4737c6dab07
Type fulltextMimetype application/pdf

Other links

Publisher's full texthttp://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13557/full

Search in DiVA

By author/editor
Whitehouse, Martin J.
By organisation
Department of Geology
In the same journal
Environmental Microbiology Reports
Microbiology

Search outside of DiVA

GoogleGoogle Scholar
Total: 4 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 6 hits
ReferencesLink to record
Permanent link

Direct link