Change search
ReferencesLink to record
Permanent link

Direct link
One-night sleep deprivation induces changes in the DNA methylation and serum activity indices of stearoyl-CoA desaturase in young healthy men
Univ Iceland, Fac Med, Dept Physiol, Vatnsmyrarvegur 16, IS-101 Reykjavik, Iceland..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Functional Pharmacology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Functional Pharmacology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Functional Pharmacology.
2016 (English)In: Lipids in Health and Disease, ISSN 1476-511X, E-ISSN 1476-511X, Vol. 15, 134Article in journal (Refereed) Published
Abstract [en]

Background: Sleep deprivation has been associated with obesity among adults, and accumulating data suggests that stearoyl-CoA desaturase 1 (SCD1) expression has a relevant impact on fatty acid (FA) composition of lipid pools and obesity. The aim of this study was to investigate the effect of one-night total sleep deprivation (TSD) on DNA methylation in the 5'-prime region of SCD1, and whether detected changes in DNA methylation are associated with SCD activity indices (product to precursor FA ratios; 16:In-7/16:0 and 18:IN-9/18:0) derived from serum phospholipids (PL). Methods: Sixteen young, normal-weight, healthy men completed two study sessions, one with one-night TSD and one with one-night normal sleep (NS). Sleep quality and length was assessed by polysomnography, and consisted of electroencephalography, electrooculography, and electromyography. Fasting whole blood samples were collected on the subsequent morning for analysis of DNA methylation and FA5 in serum PL. Linear regression analyses were performed to assess the association between changes in DNA methylation and SCD activity indices. Results: Three CpG sites close to the transcription start site (TSS) of SCD1 (cg00954566, cg24503796, cg14089512) were significantly differentially methylated in dependency of sleep duration (-log(10)P-value > 1.3). Both SCD-16 and SCD-18 activity indices were significantly elevated (P < 0.05) following one-night TSD, and significantly associated with DNA methylation changes of the three mentioned probes in the 5' region of SCD1. Conclusion: Our results suggest a relevant link between TSD, hepatic SCD1 expression and de-novo fatty acid synthesis via epigenetically driven regulatory mechanisms.

Place, publisher, year, edition, pages
2016. Vol. 15, 134
Keyword [en]
DNA methylation, Fatty acid composition, Monounsaturated fatty acids, Sleep condition, Stearoyl-CoA desaturase
National Category
Nutrition and Dietetics Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:uu:diva-307881DOI: 10.1186/s12944-016-0309-1ISI: 000382362400001PubMedID: 27562731OAI: oai:DiVA.org:uu-307881DiVA: diva2:1048839
Funder
Swedish Research Council
Available from: 2016-11-22 Created: 2016-11-22 Last updated: 2016-11-22Bibliographically approved

Open Access in DiVA

fulltext(506 kB)25 downloads
File information
File name FULLTEXT01.pdfFile size 506 kBChecksum SHA-512
22442b1a5c25a78fb8e1343565706c36c72fd6e197b41c29f8276a0d651e3ed978df69e2e0b67cd27ffeaa457f1b4f860ce627b0d9dcfbd3455cff623d1c7d11
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Nilsson, Emil KarlMwinyi, JessicaSchiöth, Helgi Birgir
By organisation
Functional Pharmacology
In the same journal
Lipids in Health and Disease
Nutrition and DieteticsMedical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Search outside of DiVA

GoogleGoogle Scholar
Total: 25 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 22 hits
ReferencesLink to record
Permanent link

Direct link