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Functional Profiling Of Metabolic Regulation In Marine Bacteria
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science. (Marine Microbial Ecology)
2016 (English)Doctoral thesis, monograph (Other academic)
Abstract [en]

Oceans are powered by active, metabolically diverse microorganisms, which are important in regulating biogeochemical cycles on Earth. Most of the ocean surface is often limited by nutrients, influencing bacterial growth and activities. Bacterial adaptation to fluctuating environmental conditions involves extensive reprogramming, and redirection of bacterial metabolism and physiology. In this thesis, I investigated the molecular mechanisms of bacterial adaptation strategies to sustain their growth and survival, focusing on the regulation of gene and protein expression in heterotrophic marine bacteria.

Comparative proteomics analyses of the growth and non-growth conditions, uncovered central adaptations that marine bacteria employ to allow them to change their metabolism to support exponential growth in response to nutrients and to readjust to stationary phase under nutrient limitation. Our results highlight that during nutrient rich conditions three distinct bacteria lineages have great similarities in their proteome. On the other hand, we observed pronounced differences in behavior between taxa during stationary phase.

Analyses of the proteorhodopsin containing bacterium Vibrio sp. AND4 during starvation showed that significantly improved survival in the light compared to darkness. Notably, proteins involved in promoting cell vitality and survival had higher relative abundance under light. In contrast, cells in the dark need to degrade their endogenous resources to support their basic cellular demands under starvation. Thus, light strongly influences how PR-containing bacteria organize their molecular composition in response to starvation.

Study of alternative energy generation metabolisms in the Alphaproteobacteria Phaeobacter sp. MED193 showed that the addition of thiosulfate enhanced the bacterial growth yields. Concomitantly, inorganic sulfur oxidation gene expression increased with thiosulfate compared to controls. Moreover, thiosulfate stimulated protein synthesis and anaplerotic CO2 fixation. These findings imply that this bacterium could use their lithotrophic potential to gain additional energy from sulfur oxidation for both improving their growth and survival.

This thesis concludes that analyses in model organisms under defined growth conditions gives invaluable knowledge about the regulatory networks and physiological strategies that ensure the growth and survival of heterotrophic bacteria. This is critically important for interpreting bacterial responses to dynamic environmental changes.

Moreover, these analyses are crucial for understanding genetic and proteomic responses in microbial communities or uncultivated organisms in terms of defining ecological niches of planktonic bacteria

Place, publisher, year, edition, pages
Växjö: Linnaeus University Press, 2016. , 152 p.
Series
Linnaeus University Dissertations, 245/2016Linnaeus University Dissertations
Keyword [en]
marine microbiology, physiology, heterotrophic bacteria, adaptive strategies, survival, proteomics, growth phase, proteorhodopsin, inorganic sulphur oxidation, anaplerotic CO2 fixation
National Category
Microbiology
Research subject
Natural Science, Environmental Science
Identifiers
URN: urn:nbn:se:lnu:diva-58257ISBN: ISBN: 978-91-88357-047-6OAI: oai:DiVA.org:lnu-58257DiVA: diva2:1048832
Public defence
2016-05-20, Fullriggaren, Kalmar, 09:30 (English)
Opponent
Supervisors
Available from: 2016-11-24 Created: 2016-11-22 Last updated: 2016-11-24Bibliographically approved

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Muthusamy, Saraladevi
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Department of Biology and Environmental Science
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