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Massive and parallel expression profiling using microarrayed single-cell sequencing
KTH Royal Inst Technol, Sch Biotechnol, Div Gene Technol, Sci Life Lab, SE-11428 Stockholm, Sweden..
Karolinska Inst, Dept Cell & Mol Biol, SE-17177 Stockholm, Sweden..
KTH Royal Inst Technol, Sch Biotechnol, Div Gene Technol, Sci Life Lab, SE-11428 Stockholm, Sweden..
Karolinska Inst, Dept Cell & Mol Biol, SE-17177 Stockholm, Sweden..
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2016 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 7, 13182Article in journal (Refereed) Published
Abstract [en]

Single-cell transcriptome analysis overcomes problems inherently associated with averaging gene expression measurements in bulk analysis. However, single-cell analysis is currently challenging in terms of cost, throughput and robustness. Here, we present a method enabling massive microarray-based barcoding of expression patterns in single cells, termed MASC-seq. This technology enables both imaging and high-throughput single-cell analysis, characterizing thousands of single-cell transcriptomes per day at a low cost (0.13 USD/cell), which is two orders of magnitude less than commercially available systems. Our novel approach provides data in a rapid and simple way. Therefore, MASC-seq has the potential to accelerate the study of subtle clonal dynamics and help provide critical insights into disease development and other biological processes.

Place, publisher, year, edition, pages
2016. Vol. 7, 13182
National Category
Cancer and Oncology
Identifiers
URN: urn:nbn:se:uu:diva-307533DOI: 10.1038/ncomms13182ISI: 000385549400001PubMedID: 27739429OAI: oai:DiVA.org:uu-307533DiVA: diva2:1047373
Funder
Knut and Alice Wallenberg FoundationSwedish Cancer SocietySwedish Foundation for Strategic Research Swedish Research Council, 621-2014-5503Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Available from: 2016-11-17 Created: 2016-11-17 Last updated: 2016-11-17Bibliographically approved

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