A surprisingly poor correlation between in vitro and in vivo testing of biomaterials for bone regeneration: Results of a multicentre analysis
2016 (English)In: European Cells and Materials, ISSN 1473-2262, E-ISSN 1473-2262, Vol. 31, 312-322 p.Article in journal (Refereed) Published
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Orthopaedics. Univ Southampton, Inst Dev Sci, Ctr Human Dev Stem Cells & Regenerat, Bone & Joint Res Grp, Southampton SO9 5NH, Hants, England.
Dawson, J. I.
Univ Southampton, Inst Dev Sci, Ctr Human Dev Stem Cells & Regenerat, Bone & Joint Res Grp, Southampton SO9 5NH, Hants, England..
Swiss Fed Inst Technol Zurich ETHZ, Inst Biomech, Vladimir Prelog Weg 3, CH-8093 Zurich, Switzerland.;Eindhoven Univ Technol, Dept Biomed Engn, POB 513, NL-5600 MB Eindhoven, Netherlands.;Eindhoven Univ Technol, Inst Complex Mol Syst, POB 513, NL-5600 MB Eindhoven, Netherlands..
Swiss Fed Inst Technol Zurich ETHZ, Inst Biomech, Vladimir Prelog Weg 3, CH-8093 Zurich, Switzerland..
New regenerative materials and approaches need to be assessed through reliable and comparable methods for rapid translation to the clinic. There is a considerable need for proven in vitro assays that are able to reduce the burden on animal testing, by allowing assessment of biomaterial utility predictive of the results currently obtained through in vivo studies. The purpose of this multicentre review was to investigate the correlation between existing in vitro results with in vivo outcomes observed for a range of biomaterials. Members from the European consortium BioDesign, comprising 8 universities in a European multicentre study, provided data from 36 in vivo studies and 47 in vitro assays testing 93 different biomaterials. The outcomes of the in vitro and in vivo experiments were scored according to commonly recognised measures of success relevant to each experiment. The correlation of in vitro with in vivo scores for each assay alone and in combination was assessed. A surprisingly poor correlation between in vitro and in vivo assessments of biomaterials was revealed indicating a clear need for further development of relevant in vitro assays. There was no significant overall correlation between in vitro and in vivo outcome. The mean in vitro scores revealed a trend of covariance to in vivo score with 58 %. The inadequacies of the current in vitro assessments highlighted here further stress the need for the development of novel approaches to in vitro biomaterial testing and validated pre-clinical pipelines.
Place, publisher, year, edition, pages
2016. Vol. 31, 312-322 p.
in vivo, in vitro, correlation, biomaterials, multicentre study
IdentifiersURN: urn:nbn:se:uu:diva-306778DOI: 10.22203/eCM.v031a20ISI: 000384895100020PubMedID: 27215739OAI: oai:DiVA.org:uu-306778DiVA: diva2:1047019
FunderEU, FP7, Seventh Framework Programme, 262948