Change search
ReferencesLink to record
Permanent link

Direct link
Evaluation of isobutanol tolerance and gene expression in four different Saccharomyces cerevisiae strains for the development of bio-butanol production
Linköping University, Department of Physics, Chemistry and Biology, Biology.
2016 (English)Independent thesis Basic level (degree of Bachelor), 10,5 credits / 16 HE creditsStudent thesis
Abstract [en]

Today, most transportation fuels are derived from crude oil. However, fossil fuels are limited resources and contribute to climate change, and are therefore not considered as sustainable. Biofuels are highly relevant candidates for replacing fossil fuels and research has gone into butanol as a biofuel. It has a high energy density, is less hygroscopic and can be blended up to 85% with gasoline. The yeast Saccharomyces cerevisiae is considered a good host for bio- butanol production; it produces small amounts of isobutanol naturally through the Ehrlich pathway, is easy to manipulate genetically and can therefore be engineered to produce higher titres of butanol. End-product toxicity, however, is a problem that needs to be solved to make butanol production in S. cerevisiae more effective, since the organism cannot tolerate higher concentrations of butanol than 2%. Four different S. cerevisiae strains were cultivated in 1.5%, 2%, 3% and 4% isobutanol by spot tests and in liquid media to evaluate their tolerance. Gene expression was measured for genes RPN4, RTG1 and ILV2 to examine their up-regulation and relevance in butanol tolerance. S. cerevisiae strain Saflager 34/70 was determined as the most tolerant strain and was able to grow in 2% liquid isobutanol and 3% isobutanol on agar plates. A three-fold up-regulation of RPN4, a transcription factor involved in the regulation of proteasome gene expression, was observed. These results contribute to the progress of genetic engineering of butanol host organisms, which is needed to create a more effective production of butanol as a biofuel. 

Place, publisher, year, edition, pages
2016. , 22 p.
Keyword [en]
Biofuels, Isobutanol, Saccharomyces cerevisiae, Tolerance, RPN4, RTG1, ILV2
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-132314ISRN: LITH-IFM-G-EX--16/3187--SEOAI: oai:DiVA.org:liu-132314DiVA: diva2:1040920
Subject / course
Biology
Supervisors
Examiners
Available from: 2016-10-31 Created: 2016-10-30 Last updated: 2016-10-31Bibliographically approved

Open Access in DiVA

fulltext(1246 kB)13 downloads
File information
File name FULLTEXT01.pdfFile size 1246 kBChecksum SHA-512
5b2558e5c550083f9756ecf85e800b7100f9d66cb6e42f1cb29e0c4cc58576c12e32ab104bbf22298e658ecfac3e69b56433498cd0b93d4db3e5cd3f3009e3bb
Type fulltextMimetype application/pdf

Search in DiVA

By author/editor
Heinrup, Rebecka
By organisation
Biology
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 13 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 101 hits
ReferencesLink to record
Permanent link

Direct link