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Efficient identification of flavones, flavanones and their glycosides in routine analysis via off-line combination of sensitive NMR and HPLC experiments
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Functional Pharmacology. Graz Univ, Dept Pharmacognosy, Inst Pharmaceut Sci, Univ Pl 4, A-8010 Graz, Austria.ORCID iD: 0000-0002-0066-8217
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström. Graz Univ, Dept Pharmaceut Chem, Inst Pharmaceut Sci, Univ Pl 1, A-8010 Graz, Austria.
Graz Univ, Dept Pharmacognosy, Inst Pharmaceut Sci, Univ Pl 4, A-8010 Graz, Austria.
Graz Univ, Dept Pharmacognosy, Inst Pharmaceut Sci, Univ Pl 4, A-8010 Graz, Austria.
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2017 (English)In: Food Chemistry, ISSN 0308-8146, E-ISSN 1873-7072, Vol. 218, 600-609 p.Article in journal (Refereed) Published
Abstract [en]

We present a standardized, straightforward and efficient approach applicable in routine analysis of flavonoids combining sensitive NMR and HPLC experiments. The determination of the relative configuration of sugar moieties usually requires the acquisition of 13C NMR shift values. We use a combination of HPLC and sensitive NMR experiments (1D-proton, 2D-HSQC) for the unique identification of known flavones, flavanones, flavonols and their glycosides. Owing to their broad range of polarity, we developed HPLC and UHPLC methods (H2O/MeOH/MeCN/HCOOH) which we applied and validated by analyzing 46 common flavones and flavanones and exemplified for four plant extracts. A searchable data base is provided with full data comprising complete proton and carbon resonance assignments, expansions of HSQC-spectra, HPLC parameters (retention time, relative retention factor), UV/Vis and mass spectral data of all compounds, which enables a rapid identification and routine analysis of flavones and flavanones from plant extracts and other products in nutrition and food chemistry.

Place, publisher, year, edition, pages
2017. Vol. 218, 600-609 p.
Keyword [en]
NMR, Flavones, Flavanones, HPLC, MS, Data base
National Category
Analytical Chemistry
URN: urn:nbn:se:uu:diva-306576DOI: 10.1016/j.foodchem.2016.09.077ISI: 000386409700078PubMedID: 27719955OAI: diva2:1040830
Available from: 2016-10-28 Created: 2016-10-28 Last updated: 2016-12-01Bibliographically approved

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Blunder, MartinaOrthaber, Andreas
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Functional PharmacologyDepartment of Chemistry - Ångström
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