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Biocatalysis on the surface of Escherichia coli: melanin pigmentation of the cell exterior
KTH, School of Biotechnology (BIO), Industrial Biotechnology. (Gen Larsson)ORCID iD: 0000-0002-3314-6060
KTH, School of Biotechnology (BIO), Industrial Biotechnology. (Gen Larsson)
KTH, School of Biotechnology (BIO), Industrial Biotechnology. (Gen Larsson)
KTH, School of Biotechnology (BIO), Industrial Biotechnology.
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2016 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 6, 36117Article in journal (Refereed) Published
Abstract [en]

Today, it is considered state-of-the-art to engineer living organisms for various biotechnology applications. Even though this has led to numerous scientific breakthroughs, the enclosed interior of bacterial cells still restricts interactions with enzymes, pathways and products due to the mass-transfer barrier formed by the cell envelope. To promote accessibility, we propose engineering of biocatalytic reactions and subsequent product deposition directly on the bacterial surface. As a proof-of-concept, we used the AIDA autotransporter vehicle for Escherichia coli surface expression of tyrosinase and fully oxidized externally added tyrosine to the biopolymer melanin. This resulted in a color change and creation of a black cell exterior. The capture of ninety percent of a pharmaceutical wastewater pollutant followed by regeneration of the cell bound melanin matrix through a simple pH change, shows the superior function and facilitated processing provided by the surface methodology. The broad adsorption spectrum of melanin could also allow removal of other micropollutants.

Place, publisher, year, edition, pages
Nature Publishing Group, 2016. Vol. 6, 36117
Keyword [en]
Expression, Autotransporter, Purification, Tyrosinase, Proteins, Enzymes, Design, Gene
National Category
Bioprocess Technology
Research subject
Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-194418DOI: 10.1038/srep36117ISI: 000386212200001ScopusID: 2-s2.0-84992597799OAI: oai:DiVA.org:kth-194418DiVA: diva2:1040309
Funder
Swedish Research Council
Note

QC 20161031

Available from: 2016-10-27 Created: 2016-10-27 Last updated: 2016-11-17Bibliographically approved

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Gustavsson, MartinHörnström, DavidLundh, SusannaBelotserkovsky, JaroslavLarsson, Gen
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