Change search
ReferencesLink to record
Permanent link

Direct link
Impact of the supramolecular structure of cellulose on the efficiency of enzymatic hydrolysis
RISE, Innventia.
RISE, Innventia.
RISE, Innventia.
2015 (English)In: Biotechnology for Biofuels, ISSN 1754-6834, E-ISSN 1754-6834, Vol. 8, no 1Article in journal (Refereed) PublishedText
Abstract [en]

Background: The efficiency of enzymatic hydrolysis is reduced by the structural properties of cellulose. Although efforts have been made to explain the mechanism of enzymatic hydrolysis of cellulose by considering the interaction of cellulolytic enzymes with cellulose or the changes in the structure of cellulose during enzymatic hydrolysis, the process of cellulose hydrolysis is not yet fully understood. We have analysed the characteristics of the complex supramolecular structure of cellulose on the nanometre scale in terms of the spatial distribution of fibrils and fibril aggregates, the accessible surface area and the crystallinity during enzymatic hydrolysis. Influence of the porosity of the substrates and the hydrolysability was also investigated. All cellulosic substrates used in this study contained more than 96% cellulose. Results: Conversion yields of six cellulosic substrates were as follows, in descending order: nano-crystalline cellulose produced from never-dried soda pulp (NCC-OPHS-ND)∈>∈never-dried soda pulp (OPHS-ND)∈>∈dried soda pulp (OPHS-D)∈>∈Avicel∈>∈cotton treated with sodium hydroxide (cotton∈+∈NaOH)∈>∈cotton. Conclusions: No significant correlations were observed between the yield of conversion and supramolecular characteristics, such as specific surface area (SSA) and lateral fibril dimensions (LFD). A strong correlation was found between the average pore size of the starting material and the enzymatic conversion yield. The degree of crystallinity was maintained during enzymatic hydrolysis of the cellulosic substrates, contradicting previous explanations of the increasing crystallinity of cellulose during enzymatic hydrolysis. Both acid and enzymatic hydrolysis can increase the LFD, but no plausible mechanisms could be identified. The sample with the highest initial degree of crystallinity, NCC-OPHS-ND, exhibited the highest conversion yield, but this was not accompanied by any change in LFD, indicating that the hydrolysis mechanism is not based on lateral erosion

Place, publisher, year, edition, pages
2015. Vol. 8, no 1
National Category
Paper, Pulp and Fiber Technology
URN: urn:nbn:se:ri:diva-763DOI: 10.1186/s13068-015-0236-9ScopusID: 2-s2.0-84927641545OAI: diva2:1039715

cited By 5

Available from: 2016-10-25 Created: 2016-08-03 Last updated: 2016-10-25Bibliographically approved

Open Access in DiVA

fulltext(1094 kB)13 downloads
File information
File name FULLTEXT01.pdfFile size 1094 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full textScopus
By organisation
In the same journal
Biotechnology for Biofuels
Paper, Pulp and Fiber Technology

Search outside of DiVA

GoogleGoogle Scholar
Total: 13 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 29 hits
ReferencesLink to record
Permanent link

Direct link