Change search
ReferencesLink to record
Permanent link

Direct link
Transdifferentiation and Proliferation in Two Distinct Hemocyte Lineages in Drosophila melanogaster Larvae after Wasp Infection
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Institute of Biosciences and Medical Technology, BioMediTech, University of Tampere, Tampere, Finland.
Show others and affiliations
2016 (English)In: PLoS Pathogens, ISSN 1553-7366, E-ISSN 1553-7374, Vol. 12, no 7, e1005746Article in journal (Refereed) Published
Abstract [en]

Cellular immune responses require the generation and recruitment of diverse blood cell types that recognize and kill pathogens. In Drosophila melanogaster larvae, immune-inducible lamellocytes participate in recognizing and killing parasitoid wasp eggs. However, the sequence of events required for lamellocyte generation remains controversial. To study the cellular immune system, we developed a flow cytometry approach using in vivo reporters for lamellocytes as well as for plasmatocytes, the main hemocyte type in healthy larvae. We found that two different blood cell lineages, the plasmatocyte and lamellocyte lineages, contribute to the generation of lamellocytes in a demand-adapted hematopoietic process. Plasmatocytes transdifferentiate into lamellocyte-like cells in situ directly on the wasp egg. In parallel, a novel population of infection-induced cells, which we named lamelloblasts, appears in the circulation. Lamelloblasts proliferate vigorously and develop into the major class of circulating lamellocytes. Our data indicate that lamellocyte differentiation upon wasp parasitism is a plastic and dynamic process. Flow cytometry with in vivo hemocyte reporters can be used to study this phenomenon in detail.

Place, publisher, year, edition, pages
Public library science , 2016. Vol. 12, no 7, e1005746
National Category
Microbiology in the medical area Immunology in the medical area
URN: urn:nbn:se:umu:diva-126760DOI: 10.1371/journal.ppat.1005746ISI: 000383366400030OAI: diva2:1037784
Available from: 2016-10-18 Created: 2016-10-13 Last updated: 2016-10-18Bibliographically approved

Open Access in DiVA

fulltext(7466 kB)9 downloads
File information
File name FULLTEXT01.pdfFile size 7466 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Anderl, InesHultmark, Dan
By organisation
Department of Molecular Biology (Faculty of Medicine)
In the same journal
PLoS Pathogens
Microbiology in the medical areaImmunology in the medical area

Search outside of DiVA

GoogleGoogle Scholar
Total: 9 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 38 hits
ReferencesLink to record
Permanent link

Direct link