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Antibacterial effects of Lactobacillus and bacteriocin PLNC8 alpha beta on the periodontal pathogen Porphyromonas gingivalis
University of Örebro, Sweden.
University of Örebro, Sweden; PEAS Institute AB, Soderleden 1, Linkoping, Sweden.
Linköping University, Department of Physics, Chemistry and Biology, Molecular Physics. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Physics, Chemistry and Biology, Molecular Physics. Linköping University, Faculty of Science & Engineering.
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2016 (English)In: BMC Microbiology, ISSN 1471-2180, E-ISSN 1471-2180, Vol. 16, no 188Article in journal (Refereed) Published
Abstract [en]

Background: The complications in healthcare systems associated with antibiotic-resistant microorganisms have resulted in an intense search for new effective antimicrobials. Attractive substances from which novel antibiotics may be developed are the bacteriocins. These naturally occurring peptides are generally considered to be safe and efficient at eliminating pathogenic bacteria. Among specific keystone pathogens in periodontitis, Porphyromonas gingivalis is considered to be the most important pathogen in the development and progression of chronic inflammatory disease. The aim of the present study was to investigate the antimicrobial effects of different Lactobacillus species and the two-peptide bacteriocin PLNC8 alpha beta on P. gingivalis. Results: Growth inhibition of P. gingivalis was obtained by viable Lactobacillus and culture media from L. plantarum NC8 and 44048, but not L. brevis 30670. The two-peptide bacteriocin from L. plantarum NC8 (PLNC8 alpha beta) was found to be efficient against P. gingivalis through binding followed by permeabilization of the membranes, using Surface plasmon resonance analysis and DNA staining with Sytox Green. Liposomal systems were acquired to verify membrane permeabilization by PLNC8 alpha beta. The antimicrobial activity of PLNC8 alpha beta was found to be rapid (1 min) and visualized by TEM to cause cellular distortion through detachment of the outer membrane and bacterial lysis. Conclusion: Soluble or immobilized PLNC8 alpha beta bacteriocins may be used to prevent P. gingivalis colonization and subsequent pathogenicity, and thus supplement the host immune system against invading pathogens associated with periodontitis.

Place, publisher, year, edition, pages
BIOMED CENTRAL LTD , 2016. Vol. 16, no 188
Keyword [en]
Periodontitis; P. gingivalis; Lactobacillus; Bacteriocin; PLNC8
National Category
Microbiology
Identifiers
URN: urn:nbn:se:liu:diva-131904DOI: 10.1186/s12866-016-0810-8ISI: 000383422500001PubMedID: 27538539OAI: oai:DiVA.org:liu-131904DiVA: diva2:1034869
Note

Funding Agencies|Swedish Heart-Lung Foundation; Foundation of Magnus Bergvall; Foundation of Olle Engkvist; Knowledge Foundation, Sweden

Available from: 2016-10-13 Created: 2016-10-11 Last updated: 2016-11-07

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Sandén, CamillaSvärd, AnnaAili, Daniel
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