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Biocompatible colloidal dispersions for cell tracking
2010 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Due to their unique properties, magnetic nanoparticles (NPs) have large potential to be used for diagnostic and therapeutic applications in biomedics, and in this thesis NPs were to be synthesized with the objective of using them for cell tracking – monitoring of the in vivo migration of injected cells in the body, and possibly immobilization of these injected cells, being made possible by internalization of NPs in the cells in vitro. In order for the NP dispersions to be successful for this application, they needed to be non-toxic to cells and stable in water at physiological pH. They also needed to possess certain magnetic properties, in order for them to enhance the contrast when used for MRI, acting as so-called contrast agents. Besides this, they needed to be taken up by cells in vitro to a high extent, while the cells remained healthy. The cell uptake was to be assessed with Prussian Blue, a substance that turns blue in the presence of iron, and therefore stainability of the particles with this substance was another requirement. Today, the contrast agents under clinical use still have a great potential for improvement, and the objective was for the synthesized NP dispersions to show advantages over a commercially available contrast agent. Three methods were tested to render NPs stable in water, of which one was proven successful, in terms of the NPs to be colloidally stable in water at physiological pH, as well as the dispersions to show no cytotoxicity and higher contrast enhancement for MRI than what did the commercial contrast agent. Also, a modification of the method was proposed to obtain NP dispersions that further enhance the contrast.

Place, publisher, year, edition, pages
2010.
Keyword [en]
Physics Chemistry Maths, magnetic, nanoparticles, MRI, cell tracking
Keyword [sv]
Fysik, Kemi, Matematik
Identifiers
URN: urn:nbn:se:ltu:diva-48786ISRN: LTU-EX--10/065--SELocal ID: 636aee42-16c1-4e2a-aa24-de53a8a3dcb3OAI: oai:DiVA.org:ltu-48786DiVA: diva2:1022130
Subject / course
Student thesis, at least 30 credits
Educational program
Materials Engineering, master's level
Examiners
Note

Validerat; 20101217 (root)

Available from: 2016-10-04 Created: 2016-10-04 Last updated: 2017-02-16Bibliographically approved

Open Access in DiVA

The full text will be freely available from 2020-03-31 00:00
Available from 2020-03-31 00:00

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CiteExportLink to record
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Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
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  • asciidoc
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