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The infectious particle of insect-borne totivirus-like Omono River virus has raised ridges and lacks fibre complexes
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.
Natl Inst Physiol Sci, Okazaki, Aichi, Japan..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.
Natl Inst Infect Dis, Dept Med Entomol, Tokyo, Japan..
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2016 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 6, 33170Article in journal (Refereed) Published
Abstract [en]

Omono River virus (OmRV) is a double-stranded RNA virus isolated from Culex mosquitos, and it belongs to a group of unassigned insect viruses that appear to be related to Totiviridae. This paper describes electron cryo-microscopy (cryoEM) structures for the intact OmRV virion to 8.9 angstrom resolution and the structure of the empty virus-like-particle, that lacks RNA, to 8.3 angstrom resolution. The icosahedral capsid contains 120-subunits and resembles another closely related arthropod-borne totivirus-like virus, the infectious myonecrosis virus (IMNV) from shrimps. Both viruses have an elevated plateau around their icosahedral 5-fold axes, surrounded by a deep canyon. Sequence and structural analysis suggests that this plateau region is mainly composed of the extended C-terminal region of the capsid proteins. In contrast to IMNV, the infectious form of OmRV lacks extensive fibre complexes at its 5-fold axes as directly confirmed by a contrast-enhancement technique, using Zernike phase-contrast cryo-EM. Instead, these fibre complexes are replaced by a short "plug" structure at the five-fold axes of OmRV. OmRV and IMNV have acquired an extracellular phase, and the structures at the five-fold axes may be significant in adaptation to cell-to-cell transmission in metazoan hosts.

Place, publisher, year, edition, pages
2016. Vol. 6, 33170
National Category
URN: urn:nbn:se:uu:diva-304148DOI: 10.1038/srep33170ISI: 000382864800001PubMedID: 27616740OAI: diva2:1014955
Swedish Research Council, 62820081109 822-2010-6157 822-2012-5260 828-2012-108Knut and Alice Wallenberg Foundation, KAW-2011.081EU, European Research Council, ERC-291602The Swedish Foundation for International Cooperation in Research and Higher Education (STINT), JA2014-5721Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Available from: 2016-10-03 Created: 2016-10-03 Last updated: 2016-10-03Bibliographically approved

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