Hypoxia on a chip: a novel approach for patch-clamp studies in a microfluidic system with full oxygen control
2012 (English)In: World Congress on Medical Physics and Biomedical Engineering, May 26-31, 2012, Beijing, China / [ed] Mian Long, Berlin: Encyclopedia of Global Archaeology/Springer Verlag, 2012, 313-316 p.Conference paper (Refereed)
Here a new approach to perform patch-clamp investigations under anoxic and normoxic conditions on nerve cells from Sprague Dawley rats is presented. A patch-clamp micropipette is integrated within a poly-methyl methacrylate (PMMA) based microchip giving optimal control over the oxygen content and the biochemical environment. Nerve cells were trapped by optical tweezers and steered towards the patch-clamp micropipette within the micro-channels. Several experiments were performed to show proof of principle. The oxygen content within the microfluidic chamber was measured to 0.5-1.5 %. The photo-induced effect of the optical tweezers on the nerve cells was investigated in an open Petri dish. The optical trapping did not influence measurements. The microfluidic system was further tested in patch-clamp experiments. This approach showed significant advantages regarding the tuning of the oxygen content and may be used in various electrophysiological investigations of single cells demanding optimal control of the surroundings.
Place, publisher, year, edition, pages
Berlin: Encyclopedia of Global Archaeology/Springer Verlag, 2012. 313-316 p.
, IFMBE proceedings, ISSN 1680-0737 ; 39
Research subject Medical Engineering for Healthcare
IdentifiersURN: urn:nbn:se:ltu:diva-39376DOI: 10.1007/978-3-642-29305-4_84Local ID: e1b2ebc7-ed54-44d5-b957-6d495c7b3f9dISBN: 978-3-642-29304-7OAI: oai:DiVA.org:ltu-39376DiVA: diva2:1012887
World Congress on Medical Physics and Biomedical Engineering : 26/05/2012 - 31/05/2012
Validerad; 2012; 20120808 (ysko)2016-10-032016-10-032016-10-19Bibliographically approved