In tissue engineering, development of materials, which positively interact with tissues, is very important.1 In this regard, hydrogels composed of three-dimensional polymeric networks, have become more attractive materials due to their ability to absorb high water content and swells without losing their structural integrity.2 Furthermore, hydrogels need to provide physico-mechanical support for cell growth, proliferation and new tissue formation.3 However, their low mechanical properties have found one drawback4 and therefore in this study, cellulose nanocrystals (CNCBE) isolated from bioethanol residue were used as reinforcement or functional additive. The objective of this work was to develop double-crosslinked Interpenetrating Polymeric Networks (IPNs) of nanocellulose-based hydrogels on alginate and gelatin and investigate the effect of IPN processing route on physico-chemical properties of the produced hydrogels as well as their potential in soft tissue engineering. Fully bio-based porous IPN scaffolds were processed through two freeze-drying steps and crosslinked using calcium chloride and genipin. The second freeze-drying was performed to induce more pores in the structure. The morphology studies showed highly porous structure (90-97% porosity), which is beneficial for cell attachment and growth, but resulted in lower mechanical performances under compression. Addition of CNCBE and crosslinking decreased the moisture uptake while increased the compression modulus. Furthermore, the development of extracellular matrix (ECM) is expected to improve the mechanical performances after implantation.AcknowledgementsFinancial support from VINNOVA (No. 2011-02071) under MNT-ERANET project, n-POSSCOG is acknowledged. References1. Silva, S. S.; Motta, A.; Rodrigues, M. T.; Pinheiro, A. F.; Gomes, M. E.; Mano, J. F.; Reis, R. L.; Migliaresi, C. Biomacromolecules 2008, 9, 2764-2774.2. Dragan, E. S.; Perju, M. M.; Dinu, M. V. Carbohydr. Polym. 2012, 88, 270-281.3. Drury, J. L.; Mooney, D. J. Biomaterials 2003, 24, 4337-4351.4. Matricardi, P.; Di Meo, C.; Coviello, T.; Hennink, W. E.; Alhaique, F. Adv. Drug Deliv. Rev. 2013, 65, 1172-1187.
EEIGM International Conference on Advanced Materials Research : 11/06/2015 - 12/06/2015