Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Electroactive biomimetic collagen-silver nanowire composite scaffolds
Linköping University, Department of Physics, Chemistry and Biology, Molecular Physics. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Science and Technology, Physics and Electronics. Linköping University, Faculty of Science & Engineering.
University of Örebro, Sweden.
UCL, England.
Show others and affiliations
2016 (English)In: Nanoscale, ISSN 2040-3364, E-ISSN 2040-3372, Vol. 8, no 29, 14146-14155 p.Article in journal (Refereed) Published
Abstract [en]

Electroactive biomaterials are widely explored as bioelectrodes and as scaffolds for neural and cardiac regeneration. Most electrodes and conductive scaffolds for tissue regeneration are based on synthetic materials that have limited biocompatibility and often display large discrepancies in mechanical properties with the surrounding tissue causing problems during tissue integration and regeneration. This work shows the development of a biomimetic nanocomposite material prepared from self-assembled collagen fibrils and silver nanowires (AgNW). Despite consisting of mostly type I collagen fibrils, the homogeneously embedded AgNWs provide these materials with a charge storage capacity of about 2.3 mC cm(-2) and a charge injection capacity of 0.3 mC cm(-2), which is on par with bioelectrodes used in the clinic. The mechanical properties of the materials are similar to soft tissues with a dynamic elastic modulus within the lower kPa range. The nanocomposites also support proliferation of embryonic cardiomyocytes while inhibiting the growth of both Gram-negative Escherichia coli and Gram-positive Staphylococcus epidermidis. The developed collagen/AgNW composites thus represent a highly attractive bioelectrode and scaffold material for a wide range of biomedical applications.

Place, publisher, year, edition, pages
ROYAL SOC CHEMISTRY , 2016. Vol. 8, no 29, 14146-14155 p.
National Category
Biomaterials Science
Identifiers
URN: urn:nbn:se:liu:diva-131731DOI: 10.1039/c6nr02027eISI: 000381815000038PubMedID: 27385421OAI: oai:DiVA.org:liu-131731DiVA: diva2:1004482
Note

Funding Agencies|Linkoping University; Swedish Foundation for Strategic Research (SSF).

The previous status of this article was Manuscript and the working title was Collagen-Silver Nanowire Composites as Electrically Activeand Antibacterial Scaffolds for Embryonic Cardiac Cell Proliferation,

Available from: 2016-09-30 Created: 2016-09-30 Last updated: 2017-06-27Bibliographically approved
In thesis
1. Multifunctional Biomimetic Scaffolds Tailored for Cardiac Regeneration
Open this publication in new window or tab >>Multifunctional Biomimetic Scaffolds Tailored for Cardiac Regeneration
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Nature has had millions of years to perfect the structural components of the human body, but has also produced the dysfunctions that result in the cancers and diseases, which ruin that perfection. Congenital heart defects, and myocardial infarction lead to scarring that remodels heart muscle, decreasing the contractility of the heart, with profound consequences for the host. Regenerative medicine is the study of strategies to return diseased body parts to their evolutionarily optimum structure.

Nature has had millions of years to perfect the structural components of the human body, but has also produced the dysfunctions that result in the cancers and diseases, which ruin that perfection. Congenital heart defects, and myocardial infarction lead to scarring that remodels heart muscle, decreasing the contractility of the heart, with profound consequences for the host. Regenerative medicine is the study of strategies to return diseased body parts to their evolutionarily optimum structure. Cells alone cannot develop into functional tissue, as they require mechanical support and chemical signals from the extracellular matrix in order to play the correct role in the body. In order to imitate the process of tissue formation optimized by nature, scaffolds are developed as the architectural support for tissue regeneration. To mimic the elasticity and strength seen in the heart muscle is one of the major scientific conundrums of our time. The development of new multifunctional materials for scaffolds is an accepted solution for repairing failing heart muscle. In this thesis I accept the notion that endogenous cardiac cells can play a major role in addressing this problem, if we can attract them to the site of defect or injury and make them proliferate. I then proceed to show how improving on a commonly used synthetic polymer was used to develop two new biomaterials.

Polycaprolactone (PCL) fibers and sheets were studied for their ability to adsorb proteins based on their surface energies. We found that although the wettability of the PCL might be similar to positive controls for cell attachment, the large differences in surface energies may account for the increased serum protein adsorption and limit cell adhesion. The effect of fiber morphology was then investigated with respect to proliferation of mesenchymal stem cells and cardiac progenitor cells. PCL was also mechanically enhanced with thiophene conjugated single walled carbon nanotubes (T-CNT); where small concentrations of the T-CNT allowed for a 2.5 fold increase in the percentage of elongation, while retaining the proliferation profile of the cardiac progenitor cells. Although PCL is a well-known implant material, the ability to attract and adhere cardiac cells was limited. Therefore we sought to develop new biomaterials with fiber morphologies similar to the muscle fiber of the heart, but with surface energies similar to positive controls for cell attachment. Poly[2,3-bis-(3-octyloxyphenyl)quinoxaline-5,8-diyl-alt-thiophene-2,5-diyl] (TQ1) was then explored as a ribbon fiber and compared to collagen with embryonic cardiac cells, in vitro, and then implanted into rats for in vivo long term evaluations. The cardiac cells had a preferential adhesion to the TQ1 fibers, and in vivo, the fibers attracted more blood vessels and regrew functional tissue compared to the collagen controls. TQ1 fibers had the added ability to emit light in the near infrared region, which would allow for consistent tracking of the material. Although this material offered the morphological preference for the cardiac cells, it does not degrade and nor did it offer electrical conductivity. The heart muscle is an electrically active muscle. The dead tissue that is formed in the ischemic area loses its ability to  transfer the electrical signals. Hence, I have then developed collagen fibrous materials with silver nanowires to help store and inject charges that would be generated during the contraction of the heart muscle. The silver nanowires served to help carry charges whilst providing resistance to bacterial growth on the material. The collagen/silver nanowires composites were mechanically apt for the culture of embryonic cardiac cells.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2015. 80 p.
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1686
National Category
Physical Sciences Clinical Medicine
Identifiers
urn:nbn:se:liu:diva-120773 (URN)10.3384/diss.diva-120773 (DOI)978-91-7519-021-1 (ISBN)
Public defence
2015-08-28, Planck, Fysikhuset, Campus Valla, Linköping, 09:15 (English)
Opponent
Supervisors
Available from: 2015-08-24 Created: 2015-08-24 Last updated: 2017-01-11Bibliographically approved

Open Access in DiVA

fulltext(739 kB)22 downloads
File information
File name FULLTEXT01.pdfFile size 739 kBChecksum SHA-512
7efb673ff8a4d68eb4ec8497b03a2dd1f261fcae9c71064cd082499a3cebc7e1f804ca79e29ad5ae1261fd5c84f5e432e52c25db1d81db21efdabd0bf567c7fb
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Wickham, AbeniVagin, MikhailDånmark, StaffanAltimiras, JordiAili, Daniel
By organisation
Molecular PhysicsFaculty of Science & EngineeringPhysics and ElectronicsBiology
In the same journal
Nanoscale
Biomaterials Science

Search outside of DiVA

GoogleGoogle Scholar
Total: 22 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 110 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf