Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Investigation of Human Mesenchymal Stromal Cells Cultured on PLGA orPLGA/Chitosan Electrospun Nanofibers
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi. Uppsala universitet, Science for Life Laboratory, SciLifeLab. (Hilborn)ORCID-id: 0000-0001-7390-7605
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi. (Magnusson)
Vise andre og tillknytning
2015 (engelsk)Inngår i: Journal of Bioprocessing & Biotechniques, ISSN 2155-9821, Vol. 5, nr 6, artikkel-id 230Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

We compared the viability, proliferation, and differentiation of human Mesenchymal Stromal Cells (MSC)after culture on poly(lactic-co-glycolic acid) (PLGA) and PLGA/chitosan (PLGA/CH) hybrid scaffolds. We appliedconventional and emulsion electrospinning techniques, respectively, for the fabrication of the PLGA and PLGA/CH scaffolds. Electrospinning under optimum conditions resulted in an average fiber diameter of 166 ± 33 nmfor the PLGA/CH and 680 ± 175 nm for the PLGA scaffold. The difference between the tensile strength of thePLGA and PLGA/CH nanofibers was not significant, but PLGA/CH showed a significantly lower tensile modulusand elongation at break. However, it should be noted that the extensibility of the PLGA/CH was higher than thatof the nanofibrous scaffolds of pure chitosan. As expected, a higher degree of hydrophilicity was seen with PLGA/CH, as compared to PLGA alone. The biocompatibility of the PLGA and PLGA/CH scaffolds was compared usingMTS assay as well as analysis by scanning electron microscopy and confocal microscopy. The results showed thatboth scaffold types supported the viability and proliferation of human MSC, with significantly higher rates on PLGA/CH nanofibers. Nonetheless, an analysis of gene expression of MSC grown on either PLGA or PLGA/CH showed asimilar differentiation pattern towards bone, nerve and adipose tissues.

sted, utgiver, år, opplag, sider
2015. Vol. 5, nr 6, artikkel-id 230
Emneord [en]
Electrospinning; PLGA; Chitosan; Synthetic-natural scaffold; Mesenchymal stromal cells; Viability; Proliferation
HSV kategori
Identifikatorer
URN: urn:nbn:se:uu:diva-276981DOI: 10.4172/2155-9821.1000230OAI: oai:DiVA.org:uu-276981DiVA, id: diva2:903789
Tilgjengelig fra: 2016-02-16 Laget: 2016-02-16 Sist oppdatert: 2018-05-23bibliografisk kontrollert

Open Access i DiVA

fulltext(2872 kB)447 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 2872 kBChecksum SHA-512
b9f0018d455d203198fa2ab3b97e514acf8a3cb220624848ff0c07c12de760731178204ad0b4402b1cbd59095491bb320ac83eb4900d5ff93ef4d3a9b273f03f
Type fulltextMimetype application/pdf

Andre lenker

Forlagets fulltekst

Søk i DiVA

Av forfatter/redaktør
Ajalloueian, FatemehFransson, MoaTavanai, HosseinMassuni, MohammadHilborn, JönsArpanaei, AyyoobMagnusson, Peetra
Av organisasjonen

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 447 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

doi
urn-nbn

Altmetric

doi
urn-nbn
Totalt: 2369 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf