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Studies on an on/off-switchable immunosensor for troponin T
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. University of Dhaka, Bangladesh.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
Royal Institute Technology, Sweden.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
Vise andre og tillknytning
2015 (engelsk)Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 73, s. 100-107Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Regeneration is a key goal in the design of immunosensors. In this study, we report the temperature-regulated interaction of N-isopropylacrylamide (PNIPAAm) functionalised cardiac troponin T (cTnT) with anti-cTnT. Covalently bonded PNIPAAm on an anti-cTnT bioelectrode showed on/off-switchability, regeneration capacity and temperature triggered sensitivity for cTnT. Above the lower critical solution temperature (LCST), PNIPAAm provides a liphophilic microenvironment with specific volume reduction at the bioelectrode surface, making available binding space for cTnT, and facilitating analyte recognition. Computational studies provide details about the structural changes occurring at the electrode above and below the LCST. Furthermore, free energies associated with the binding of cTnT with PNIPAAm at 25 (Delta G(coil)=-6.0 Kcal/mole) and 37 degrees C (Delta G(globular)=-41.0 kcal/mole) were calculated to elucidate the interaction and stability of the antigen-antibody complex. The responsiveness of such assemblies opens the way for miniaturised, smart immuno-technologies with built-in programmable interactions of antigen-antibody upon receiving stimuli.

sted, utgiver, år, opplag, sider
Elsevier , 2015. Vol. 73, s. 100-107
Emneord [en]
On/off-switching; Troponin T; Programmable bioelectronics; Smart immunosensors
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-120853DOI: 10.1016/j.bios.2015.05.055ISI: 000358823900014PubMedID: 26056953OAI: oai:DiVA.org:liu-120853DiVA, id: diva2:849483
Merknad

Funding Agencies|Swedish Institute [00037/2014]; Initiative in Life Science Technologies (LIST); Linkoping University

Tilgjengelig fra: 2015-08-28 Laget: 2015-08-28 Sist oppdatert: 2017-12-04

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