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Perfusion Processes
KTH, Skolan för bioteknologi (BIO), Industriell bioteknologi. (Cell Technology)ORCID-id: 0000-0002-5370-4621
2015 (engelsk)Inngår i: Animal Cell Culture / [ed] Mohamed Al-Rubeai, Switzerland Springer: Springer , 2015, s. 407-443Kapittel i bok, del av antologi (Fagfellevurdert)
Abstract [en]

The interest for perfusion is increasing nowadays. This new focus has emerged from a synergy of a demand for disposable equipment and the availability of robust cell separation device, as well as the need for higher flexibility and lower investment cost. The cell separation devices mostly used today are based on filtration, i.e. alternating flow filtration, tangential flow filtration, spin-filter, or acceleration/gravity, i.e. inclined settler, centrifuge, acoustic settler. This paper gives an introduction to the basic concepts of perfusion and its practical implementation. It reviews the actual cell separation devices and describes the approaches used in the field to develop and optimize the perfusion processes.

sted, utgiver, år, opplag, sider
Switzerland Springer: Springer , 2015. s. 407-443
Serie
Cell Engineering, ISSN 1389-6946
Emneord [en]
perfusion, CHO, Chinese Hamster Ovary cells, mammalian cells, ATF, TFF, centritech, acoustic settler, inclined settler, perfusion rate
HSV kategori
Forskningsprogram
Bioteknologi
Identifikatorer
URN: urn:nbn:se:kth:diva-166112DOI: 10.1007/978-3-319-10320-4_13ISBN: 978-3-319-10320-4 (tryckt)OAI: oai:DiVA.org:kth-166112DiVA, id: diva2:809286
Merknad

QC 20150508

Tilgjengelig fra: 2015-05-01 Laget: 2015-05-01 Sist oppdatert: 2015-05-08bibliografisk kontrollert

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