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Structural studies and biosynthetic aspects of the O-antigen polysaccharide from Escherichia coli O42
Stockholm University, Faculty of Science, Department of Organic Chemistry.
Stockholm University, Faculty of Science, Department of Organic Chemistry.
2015 (English)In: Carbohydrate Research, ISSN 0008-6215, E-ISSN 1873-426X, Vol. 403, p. 174-181Article in journal (Refereed) Published
Abstract [en]

The structure of the O-antigen polysaccharide (PS) from Escherichia coli O42 has been investigated by NMR spectroscopy as the main method, which was complemented with sugar analysis, mass spectrometry, and analysis of biosynthetic information. The O-specific chain of the O-deacylated lipopolysaccharide (LPS-OH) consists of branched tetrasaccharide-glycerol repeating units joined by phosphodiester linkages. The lipid-free polysaccharide contains 0.8 equiv of O-acetyl groups per repeating unit and has the following teichoic acid-like structure: Based on biosynthetic aspects, this should also be the biological repeating unit. This O-antigen structure is remarkably similar to that of E. coli O28ac, differing only in the presence or absence, respectively, of a glucose residue at the branching point. The structural similarity explains the serological cross-reactivity observed between strains of these two serogroups, and also their almost identical O-antigen gene cluster sequences. -> 2)-(R)-Gro-(1-P-4)-beta-D-GlcpNAc-(1 -> 3)-beta-D-Galf2Ac-(1 -> 3)-alpha-D-GlcpNAc-(1 -> vertical bar a-D-Glcp-(1 -> 3)

Place, publisher, year, edition, pages
2015. Vol. 403, p. 174-181
Keywords [en]
Escherichia coli, NMR, O-Acetylation, O-Antigen
National Category
Organic Chemistry
Identifiers
URN: urn:nbn:se:su:diva-115361DOI: 10.1016/j.carres.2014.05.003ISI: 000349085900021PubMedID: 24909379OAI: oai:DiVA.org:su-115361DiVA, id: diva2:796830
Funder
Swedish Research CouncilKnut and Alice Wallenberg FoundationEU, FP7, Seventh Framework Programme
Note

AuthorCount:3;

Available from: 2015-03-20 Created: 2015-03-20 Last updated: 2022-02-23Bibliographically approved

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Fontana, CarolinaWidmalm, Göran
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