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SNP analysis by allele-specific extension in a micromachined filter chamber
KTH, Tidigare Institutioner, Bioteknologi.
KTH, Tidigare Institutioner, Bioteknologi.
KTH, Tidigare Institutioner, Bioteknologi.
KTH, Tidigare Institutioner, Bioteknologi.ORCID-id: 0000-0001-8993-048X
Visa övriga samt affilieringar
2002 (Engelska)Ingår i: BioTechniques, ISSN 0736-6205, E-ISSN 1940-9818, Vol. 32, nr 4, s. 748-754Artikel i tidskrift (Refereegranskat) Published
Ort, förlag, år, upplaga, sidor
2002. Vol. 32, nr 4, s. 748-754
Nyckelord [en]
SINGLE-NUCLEOTIDE POLYMORPHISMS, HUMAN GENOME, HYBRIDIZATION, SYSTEM, PROBES, DNA
Nationell ämneskategori
Medicinsk genetik
Identifikatorer
URN: urn:nbn:se:kth:diva-5007ISI: 000174881200006PubMedID: 11962595OAI: oai:DiVA.org:kth-5007DiVA, id: diva2:7461
Anmärkning
QC 20100929 NR 20140805Tillgänglig från: 2005-04-01 Skapad: 2005-04-01 Senast uppdaterad: 2018-01-11Bibliografiskt granskad
Ingår i avhandling
1. Microfluidic bead-based methods for DNA analysis
Öppna denna publikation i ny flik eller fönster >>Microfluidic bead-based methods for DNA analysis
2005 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

With the completion of the human genome sequencing project, attention is currently shifting toward understanding how genetic variation, such as single nucleotide polymorphism (SNP), leads to disease. To identify, understand, and control biological mechanisms of living organisms, the enormous amounts of accumulated sequence information must be coupled to faster, cheaper, and more powerful technologies for DNA, RNA, and protein analysis. One approach is the miniaturization of analytical methods through the application of microfluidics, which involves the manipulation of fluids in micrometer-sized channels. Advances in microfluidic chip technology are expected to play a major role in the development of cost-effective and rapid DNA analysis methods.

This thesis presents microfluidic approaches for different DNA genotyping assays. The overall goal is to combine the potential of the microfluidic lab-on-a-chip concept with biochemistry to develop and improve current methods for SNP genotyping. Three genotyping assays using miniaturized microfluidic approaches are addressed.

The first two assays are based on primer extension by DNA polymerase. A microfluidic device consisting of a flow-through filter chamber for handling beads with nanoliter liquid volumes was used in these studies. The first assay involved an allelespecific extension strategy. The microfluidic approach took advantage of the different reaction kinetics of matched and mismatched configurations at the 3’-ends of a primer/template complex. The second assay consisted of adapting pyrosequencing technology, a bioluminometric DNA sequencing assay based on sequencing-bysynthesis, to a microfluidic flow-through platform. Base-by-base sequencing was performed in a microfluidic device to obtain accurate SNP scoring data on nanoliter volumes. This thesis also presents the applications of monolayer of beads immobilized by microcontact printing for chip-based DNA analysis. Single-base incorporation could be detected with pyrosequencing chemistry on these monolayers.

The third assay developed is based on a hybridization technology termed Dynamic Allele-Specific Hybridization (DASH). In this approach, monolayered beads containing DNA duplexes were randomly immobilized on the surface of a microheater chip. DNA melting-curve analysis was performed by dynamically heating the chip while

simultaneously monitoring the DNA denaturation profile to determine the genotype. Multiplexing based on single-bead analysis was achieved at heating rates more than 20 times faster than conventional DASH provides.

Ort, förlag, år, upplaga, sidor
Stockholm: KTH, 2005. s. x, 52
Serie
Trita-ILA, ISSN 0281-2878 ; 0502
Nyckelord
Genetics, single nucleotide polymorphism, DNA analysis, SNP, microfluidics, pyrosequencing, beads, lab on a chip, hybridization, DASH, microsystem, micro totat analysis system, allele-specific extension, DASH, microcontact printing, Genetik
Nationell ämneskategori
Medicinsk genetik
Identifikatorer
urn:nbn:se:kth:diva-155 (URN)91-7283-992-9 (ISBN)
Disputation
2005-04-08, Q2, Osquldas v 10, KTH, 13:00
Opponent
Anmärkning
QC 20101008Tillgänglig från: 2005-04-01 Skapad: 2005-04-01 Senast uppdaterad: 2018-01-11Bibliografiskt granskad

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Ahmadian, AfshinRussom, AmanAndersson, HeleneUhlén, MathiasStemme, GöranNilsson, Peter
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