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A high-performance glucose biosensor using covalently immobilised glucose oxidase on a poly(2,6-diaminopyridine)/carbon nanotube electrode
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
University of Zanjan, Iran .
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
2013 (engelsk)Inngår i: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 116, s. 801-808Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

A highly-sensitive glucose biosensor amenable to ultra-miniaturisation was fabricated by immobilisation of glucose oxidase (GOx), onto a poly(2,6-diaminopyridine)/multi-walled carbon nanotube/glassy carbon electrode (poly(2,6-DP)/MWNT/GCE). Cyclic voltammetry was used for both the electrochemical synthesis of poly-(2,6-DP) on the surface of a MWNT-modified GC electrode, and characterisation of the polymers deposited on the GC electrode. The synergistic effect of the high active surface area of both the conducting polymer, i.e., poly-(2,6-DP) and MWNT gave rise to a remarkable improvement in the electrocatalytic properties of the biosensor. The transfer coefficient (alpha), heterogeneous electron transfer rate constant and Michaelis-Menten constant were calculated to be 0.6, 4 s(-1) and 0.20 mM at pH 7.4, respectively. The GOx/poly(2,6-DP)/MWNT/GC bioelectrode exhibited two linear responses to glucose in the concentration ranging from 0.42 mu M to 8.0 mM with a correlation coefficient of 0.95, sensitivity of 52.0 mu AmM-1 cm(-2), repeatability of 1.6% and long-term stability, which could make it a promising bioelectrode for precise detection of glucose in the biological samples. (C) 2013 Elsevier B.V. All rights reserved.

sted, utgiver, år, opplag, sider
Elsevier , 2013. Vol. 116, s. 801-808
Emneord [en]
Glucose biosensor, Direct electron transfer, Glucose oxidase, MWNT/poly-(2, 6-diaminopyridine)
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Identifikatorer
URN: urn:nbn:se:liu:diva-102852DOI: 10.1016/j.talanta.2013.07.068ISI: 000328176000114OAI: oai:DiVA.org:liu-102852DiVA, id: diva2:683823
Merknad

Funding Agencies|European Commission|PIIF-GA-2009-254955|LIST - Linkoping University||Zanjan University Research Council||

Tilgjengelig fra: 2014-01-07 Laget: 2014-01-02 Sist oppdatert: 2017-12-06

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