Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Conditioned media from macrophages of M1, but not M2 phenotype, inhibit the proliferation of the colon cancer cell lines HT-29 and CACO-2
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Health Sciences (from 2013).
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Health Sciences (from 2013).ORCID iD: 0000-0002-3813-9548
School of Health and Medical Sciences, Örebro University.
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Health Sciences (from 2013).
2014 (English)In: International Journal of Oncology, ISSN 1019-6439, Vol. 44, no 2, p. 385-392Article in journal (Refereed) Published
Abstract [en]

Solid tumors are infiltrated by stroma cells including macrophages and these cells can affect tumor growth, metastasis and angiogenesis. We have investigated the effects of conditioned media (CM) from different macrophages on the proliferation of the colon cancer cell lines HT-29 and CACO-2. CM from THP-1 macrophages and monocyte-derived human macrophages of the M1 phenotype, but not the M2 phenotype, inhibited proliferation of the tumor cells in a dose-dependent manner. Lipopolysaccaharide and interferon γ was used for differentiation of macrophages towards the M1 phenotype and CM were generated both during differentiation (M1DIFF) and after differentiation (M1). M1 and M1DIFF CM as well as THP-1 macrophage CM resulted in cell cycle arrest in HT-29 cells with a decrease of cells in S phase and an increase in G2/M phase. Treatment of HT-29 cells with M1DIFF, but not M1 or THP-1 macrophage CM, resulted in apoptosis of about 20% of the tumor cells and this was accompanied by lack of recovery of cell growth after removal of CM and subsequent culture in fresh media. A protein array was used to identify cytokines released from M1 and M2 macrophages. Among the cytokines released by M1 macrophages, tumor necrosis factor α and CXCL9 were tested by direct addition to HT-29 cells, but neither affected proliferation. Our results indicate that M1 macrophages inhibit colon cancer cell growth and have the potential of contributing to reducing tumor growth in vivo.

Place, publisher, year, edition, pages
Spandidos Publications , 2014. Vol. 44, no 2, p. 385-392
Keywords [en]
M1 macrophages, M2 macrophages, THP-1, colon cancer cell line, HT-29, CACO-2
National Category
Health Sciences
Research subject
Biomedical Sciences
Identifiers
URN: urn:nbn:se:kau:diva-30534DOI: 10.3892/ijo.2013.2203ISI: 000332687400005PubMedID: 24296981OAI: oai:DiVA.org:kau-30534DiVA, id: diva2:668803
Available from: 2013-12-02 Created: 2013-12-02 Last updated: 2020-01-13Bibliographically approved

Open Access in DiVA

fulltext(453 kB)3 downloads
File information
File name FULLTEXT01.pdfFile size 453 kBChecksum SHA-512
e2433fc82c60caa65a3b816f8f43027a101cfecb02494a3b5352820ea7952bdd95245feac3d002e5980346c0b5588661f6a1000f8623fa5e2de5eb2c8bb5f027
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Engström, AlexanderErlandsson, AnnDelbro, DickWijkander, Jonny
By organisation
Department of Health Sciences (from 2013)
In the same journal
International Journal of Oncology
Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 3 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 257 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf